Nitrogen application regulates antioxidant capacity and flavonoid metabolism,especially quercetin,in grape seedlings under salt stress  

作　　者：Congcong Zhang Han Wang Guojie Nai Lei Ma Xu Lu Haokai Yan Meishuang Gong YuanyuanLi Ying Lai Zhihui Pu Li Wei Guiping Chen Ping Sun Baihong Chen Shaoying Ma Sheng Li 

机构地区：[1]College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China [2]College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China [3]College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China [4]Laboratory and Practice Base Management Center,Gansu Agricultural University,Lanzhou 730070,China [5]State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4074-4092,共19页农业科学学报（英文版）

基　　金：supported by the Key Talent Project of Gansu Provincial Party Committee Organization Department Funding,China(2023RCXM23);the Industrial Support of Gansu Provincial Department of Education Funding,China(2021CYZC-55);the Key Research and Development Projects of Gansu Provincial Funding,China(21YF5NA090)。

摘　　要：Salt stress is a typical abiotic stress in plants that causes slow growth,stunting,and reduced yield and fruit quality.Fertilization is necessary to ensure proper crop growth.However,the effect of fertilization on salt tolerance in grapevine is unclear.In this study,we investigated the effect of nitrogen fertilizer(0.01 and 0.1 mol L^(-1)NH_(4)NO_(3))application on the salt(200 mmol L^(-1)NaCl)tolerance of grapevine based on physiological indices,and transcriptomic and metabolomic analyses.The results revealed that 0.01 mol L^(-1)NH_(4)NO_(3) supplementation significantly reduced the accumulation of superoxide anion(O_(2)^(-)·),enhanced the activities of superoxide dismutase(SOD)and peroxidase(POD),and improved the levels of ascorbic acid(AsA)and glutathione(GSH)in grape leaves compared to salt treatment alone.Specifically,joint transcriptome and metabolome analyses showed that the differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were significantly enriched in the flavonoid biosynthesis pathway(ko00941)and the flavone and flavonol biosynthesis pathway(ko00944).In particular,the relative content of quercetin(C00389)was markedly regulated by salt and nitrogen.Further analysis revealed that exogenous foliar application of quercetin improved the SOD and POD activities,increased the AsA and GSH contents,and reduced the H_(2)O_(2) and O_(2)^(-)·contents.Meanwhile,10 hub DEGs,which had high Pearson correlations(R^(2)>0.9)with quercetin,were repressed by nitrogen.In conclusion,all the results indicated that moderate nitrogen and quercetin application under salt stress enhanced the antioxidant system defense response,thus providing a new perspective for improving salt tolerance in grapes.

关 键 词：GRAPEVINE salt stress nitrogen multi-omics QUERCETIN antioxidant 

分 类 号：S663.1[农业科学—果树学]