A potential hyphal fusion protein complex with an important role in development and virulence interacts with autophagy-related proteins in Fusarium pseudograminearum  

作　　者：Linlin Chen Yixuan Shan Zaifang Dong Yake Zhang Mengya Peng Hongxia Yuan Yan Shi Honglian Li Xiaoping Xing 

机构地区：[1]College of Plant Protection,Henan Agricultural University,Zhengzhou 450000,China [2]National Key Laboratory of Wheat and Maize Crop Science,Zhengzhou 450000,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4093-4106,共14页农业科学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(U2004140);the Henan Provincial Science and Technology Major Project,China(221100110100)。

摘　　要：Hyphal fusion(anastomosis)is a common process serving many important functions at various developmental stages in the life cycle of ascomycetous fungi.However,the biological roles and molecular mechanisms in plant pathogenic fungi were widely unknown.In this study,a hyphal fusion protein FpHam-2 was screened from a T-DNA insertion mutant library of Fusarium pseudograminearum,and FpHam-2 interacts with another 2 hyphal fusion protein homologues FpHam-3 and FpHam-4.Each of these 3 genes deletion mutant revealed in similar defective phenotypes compared with the WT and complemented strains,including reduction in growth rate,defects in hyphal fusion and conidiation,more sensitive for cell membrane,cell wall and oxidative stress responses,and decreased in virulence.The yeast two-hybrid assay was used to identify that FpHam-2 interacts with 3 autophagy-related proteins,including FpAtg3,FpAtg28 and FpAtg33.Furthermore,FpHam-2-deletion mutant showed decreased accumulation of autophagic bodies in hypha.In conclusion,FpHam-2,FpHam-3 and FpHam-4 have an essential role for hyphal fusion and regulating the growth,conidiation and virulence in F.pseudograminearum.

关 键 词：Fusarium pseudograminearum hyphal fusion FpHam-2 VIRULENCE autophagy-related proteins 

分 类 号：S432.44[农业科学—植物病理学]