Neuropeptide signaling systems are involved in regulating thermal tolerance in the oriental fruit fly  

作　　者：Yang Yang Hongfei Li Changhao Liang Donghai He Hang Zhao Hongbo Jiang Jinjun Wang 

机构地区：[1]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Southwest University,Chongqing 400715,China [2]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400715,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4147-4160,共14页农业科学学报（英文版）

基　　金：supported by funding from the National Natural Science Foundation of China(U21A20222 and 32072491);the National Key R&D Program of China(2022YFC2601000);the 111 Project,China(B18044)and the earmarked fund for China Agriculture Research System(CARS-26)。

摘　　要：Neuropeptides and their receptors are involved in the regulation and coordination of various physiological processes in insects.Although various neuropeptides have been identified previously,the corresponding receptors remain unknown in the oriental fruit fly,Bactrocera dorsalis,an important agricultural insect pest.It is also unclear whether neuropeptide signaling systems are involved in regulating the thermal tolerance of this notorious pest.Here,we systematically identified 44 putative neuropeptide receptor genes which encode 66 protein sequences,and analyzed the spatio-temporal expression patterns of the neuropeptide ligands and their receptor genes in B.dorsalis.We also analyzed changes in their transcript accumulation in two thermo-tolerant populations(heat and cold)of B.dorsalis.The results showed that numerous neuropeptides and receptors participate in responding to thermal stresses during acclimation.In particular,the expression of short neuropeptide F(sNPF)was up-regulated in the heat-tolerant population of B.dorsalis.Moreover,proteomic data showed that sNPF was up-regulated in both thermo-tolerant populations of B.dorsalis.The functional verification based on CRISPR/Cas9 demonstrated that sNPF is involved in regulating the tolerance to thermal stresses.The results of this study enrich our knowledge on the function of neuropeptide sNPF in insects.Moreover,this study demonstrated the role of neuropeptide signaling systems in thermal adaptation,contributing to a better understanding of the rapid invasiveness of B.dorsalis around the world.

关 键 词：Bactrocera dorsalis neuropeptide receptors sNPF thermal adaptation CRISPR/Cas9 

分 类 号：S433[农业科学—农业昆虫与害虫防治]