Klebsiella as an α-tocopherol source facilitating Lactobacillus plantarum fermentation in rice straw silage  

作　　者：Cheng Zong Lu Tang Tao Shao Yu Xiao Zhongyong Huang Wanqi Jiang Jiugang Zhu Zhihao Dong Mao Li Qinhua Liu 

机构地区：[1]Institute of Ensiling and Processing of Grass,College of Agro-grassland Science,Nanjing Agricultural University,Nanjing 210095,China [2]Yunnan Provincial Key Laboratory of Animal Nutrition and Feed,Faculty of Animal Science and Technology,Yunnan AgriculturalUniversity,Kunming 650201,China [3]Tropical Crops Genetic Resource Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [4]Chongqing Sericulture Science and Technology Research Institute,Chongqing 400700,China [5]Chongqing Academy of Animal Sciences,Chongqing 402460,China

出　　处：《Journal of Integrative Agriculture》2024年第12期4186-4202,共17页农业科学学报（英文版）

基　　金：supported by the Yunnan Revitalization Talents Support Plan,China(XDYC-QNRC-2023-0408);the Yunnan Revitalization Talents Support Plan,China(XDYC-CYCX-2022-0036);the Key Research and Development Projects of Hainan Province,China(ZDYF2022XDNY153);the Natural Science Foundation of Chongqing City,China(cstc2019jcyj-msxmX0066);the Fundamental Research Funds or the Central Universities,China(KYYZ2023002)。

摘　　要：Silage serves as the indispensable diet of ruminants,the increasing mechanism of α-tocopherol during silage making is unclear.Rice straw lacks chlorophyll after harvesting the grain,this can eliminate the impact of tocopherols formed by the breakdown of chlorophyll.Here,we explored the α-tocopherol source,its influencing factor,and its relationship with dominant lactic acid bacteria in rice straw silage treated without or with different additives(sodium benzoate,Lactobacillus plantarum,cell wall degrading enzymes,the combination of L.plantarum and cell wall degrading enzymes)and vacuum times(5,8,11,and 14 s)after ensiling for 42 d.We found that the pathogenic Klebsiella was traced as the source of increasedα-tocopherol in rice straw silage.The residue air in the silo,pH value,and additive variety had impacts on Klebsiella activity,which was strongly active at levels of residue air in the silo and pH that were high.As an acidic niche creator,L.plantarum was more effective than sodium benzoate in restraining Klebsiella.Despite having a low acidity tolerance,Klebsiella was still present in rice straw silage treated with L.plantarum.The relationship between Klebsiella and L.plantarum was that Klebsiella could affordα-tocopherol to the multiplication of L.plantarum and residue capsular polysaccharide protected Klebsiella from escaping the extinction in rice straw silage.

关 键 词：Α-TOCOPHEROL KLEBSIELLA Lactobacillus plantarum rice straw silage 

分 类 号：S816.53[农业科学—饲料科学]