针刺联合刺血疗法对荨麻疹模型大鼠肥大细胞脱颗粒效应的影响  

Effect of acupuncture combined with bloodletting therapy on mast cell degranulation in urticaria rats

作　　者：杜玉茱 梁玉磊 石钰 薛玉强 刘湘 田赞 孙明新 王艳君 DU Yuzhu;LIANG Yulei;SHI Yu;XUE Yuqiang;LIU Xiang;TIAN Zan;SUN Mingxin;WANG Yanjun(College of Acupuncture-Moxibustion and Tuina,Hebei University of CM,Shijiazhuang 050200,China;Department of Rehabilitation,Affiliated Hospital of Hebei University;College of Basic Medicine,Hebei University of CM,Shijiazhuang 050200,China;Department of Dermatology,First Affiliated Hospital of Hebei University of CM,Shijiazhuang 050011;Department of Acupuncture and Moxibustion,First Affiliated Hospital of Hebei University of CM,Shijiazhuang 050011)

机构地区：[1]河北中医药大学针灸推拿学院,石家庄050200 [2]河北大学附属医院康复科 [3]河北中医药大学基础医学院,石家庄050200 [4]河北中医药大学第一附属医院,皮肤科,石家庄050011 [5]河北中医药大学第一附属医院,针灸科,石家庄050011

出　　处：《中国针灸》2024年第11期1273-1280,共8页Chinese Acupuncture & Moxibustion

基　　金：国家重点研发计划“中医药现代化研究”重点专项项目:2017YFC1703605;河北省科技厅重点研发计划项目:21377766D;河北省中医药管理局项目:2021094。

摘　　要：目的:观察针刺联合刺血疗法对荨麻疹模型大鼠血清炎性因子表达、致敏部位皮肤组织形态及肥大细胞脱颗粒情况的影响,探索其治疗荨麻疹的可能机制。方法:SPF级SD大鼠42只,随机选取6只大鼠制备致敏抗血清,剩余36只大鼠随机分为空白组、模型组、阳性药物组、针刺组、刺血组和针刺+刺血组,每组6只,采用被动皮肤过敏反应制备荨麻疹大鼠模型。阳性药物组予1 mL氯雷他定溶液(1 mg·kg^(-1))灌胃,每日1次;针刺组先予1 mL 0.9%氯化钠溶液灌胃,1 h后进行针刺干预,穴取“百会”“中脘”及双侧“曲池”“血海”,每日1次,每次15 min;刺血组先予1 mL 0.9%氯化钠溶液灌胃,1 h后进行刺血疗法,于“大椎”及双侧“膈俞”点刺放血,每日1次,每穴放血量约0.1 mL;针刺+刺血组先予1 mL 0.9%氯化钠溶液灌胃,1 h后联合采用针刺和刺血干预,每日1次。各组均于第6天开始干预,均干预2周。末次干预结束后,各组大鼠进行抗原激发。ELISA法检测大鼠血清免疫球蛋白E(IgE)、类胰蛋白酶(TPS)、白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、肿瘤坏死因子-α(TNF-α)含量,直尺测量大鼠背部致敏部位皮肤蓝斑直径,HE染色观察大鼠背部致敏部位皮肤组织形态,甲苯胺蓝染色观察大鼠背部致敏部位皮肤肥大细胞脱颗粒情况。结果:与空白组比较,模型组大鼠血清Ig E、TPS、IL-4、IL-5、TNF-α含量升高(P<0.01),背部致敏部位皮肤蓝斑直径增大(P<0.01),背部致敏部位皮肤组织肥大细胞脱颗粒率升高(P<0.01),模型组大鼠背部致敏部位皮肤组织出现明显炎性细胞浸润及真皮层水肿。与模型组比较,阳性药物组、针刺组、刺血组和针刺+刺血组大鼠血清Ig E、TPS、IL-4、IL-5、TNF-α含量降低(P<0.01,P<0.05),阳性药物组和针刺+刺血组大鼠背部致敏部位皮肤蓝斑直径减小(P<0.05),阳性药物组、针刺组、刺血组和针刺+刺血组大鼠背部致敏部位Objective To observe the effects of acupuncture combined with bloodletting on the expression of inflammatory factors in serum,the morphology of sensitized skin tissue and the mast cell degranulation in urticaria rats,and to explore the potential mechanism of this therapy for urticaria.Methods Among 42 SD rats of SPF grade,6 rats were randomly collected for the preparation of sensitized antiserum;and the rest 36 rats were randomized into a blank group,a model group,a positive drug group,an acupuncture group,a bloodletting group and a combined treatment group(acupuncture+bloodletting),6 rats in each one.The rat model of urticaria was established by passive cutaneous anaphylaxis.In the positive drug group,loratadine(1 mg·kg^(-1))by gavage was administered once a day.In the acupuncture group,1 h after gavage with 0.9%NaCl(1 mL),acupuncture was delivered at"Baihui"(GV 20),"Zhongwan"(CV 12),and bilateral"Quchi"(LI 11)and"Xuehai"(SP 10)for 15 min,once daily.In the bloodletting group,1 h after gavage with 0.9%NaCl(1 mL),bloodletting was operated at"Dazhui"(GV 14)and bilateral"Geshu"(BL 17),around 0.1 mL of bleeding volume at each point,once daily.In the combined treatment group,1 h after gavage with 0.9%NaCl(1 mL),the interventions as the acupuncture group and the bloodletting group were adopted,once daily.All the interventions started on day 6 of modeling,lasting 2 weeks.After intervention completion,antigenic stimulation was performed in the rats of each group.Using ELISA,the levels of serum immunoglobulin E(IgE),tryptase(TPS),interleukin-4(IL-4),interleukin-5(IL-5),tumor necrosis factor-α(TNF-α)were detected.The diameter of the blue spots of the sensitized skin on the back was measured with ruler in each rat.The morphology of sensitized skin tissue was observed using HE staining,and the degranulation of mast cells was observed using Toluidine blue staining.Results Compared with the blank group,in the model group,the levels of serum IgE,TPS,IL-4,IL-5 and TNF-αincreased(P<0.01),the diameter of blue spot on the sensi

关 键 词：荨麻疹 针刺 刺血 肥大细胞脱颗粒 被动皮肤过敏反应 

分 类 号：R245[医药卫生—针灸推拿学]