阿旺绵羊群体结构及毛色性状关联分析  

Association between Population Structure and Coat Color Traits in Awang Sheep

作　　者：杨志勇 孙一斐 白凤庭 赵赫 王幸栓 叶美琳 王喜宏 李建强 YANG Zhiyong;SUN Yifei;BAI Fengting;ZHAO He;WANG Xingshuan;YE Meilin;WANG Xihong;LI Jianqiang(Tianjin Jinken Animal Husbandry Group Co.,Ltd.,Tianjin 300380;Northwest A&F University,Yangling Shaanxi,712100;Tianjin Tianshi Cattle Seed Industry Co.,Ltd.,Tianjin 300409)

机构地区：[1]天津津垦牧业集团有限公司,天津300380 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]天津天食牛种业有限公司,天津300409

出　　处：《家畜生态学报》2024年第11期7-13,共7页Journal of Domestic Animal Ecology

摘　　要：利用绵羊40K SNP液相芯片,对1003只阿旺绵羊进行SNP测定,结合阿旺绵羊的毛色表型,进行毛色性状的关联分析;计算个体间亲缘关系,与藏系绵羊、蒙古系绵羊、云南绵羊进行主成分分析、系统发育树的构建、祖先分析,筛选阿旺绵羊独特的分子标记。结果表明,阿旺绵羊是藏系绵羊,与蒙古羊和云南羊有较大差异,在藏羊中也是独特的类群;全基因组关联分析分析发现了阿旺绵羊的毛色黑/褐性状与基因组上14号染色体和20号染色体上的5个SNPs显著相关,这些SNPs临近或位于2个已知基因MC1R、TCF25。通过与国内外大样本绵羊基因组的比较,筛选到8个阿旺绵羊品种特异性SNP用于品种鉴定。综上所述,阿旺绵羊从基因组上具有特异性,是藏羊的独特遗传资源。In this study,1003 Awang sheep were tested using sheep 40K SNP liquid chip,and genome wide association analysis of hair color traits was carried out combined with the hair color phenotype of Awang sheep.The genetic relationship among individuals was calculated,and then principal component analysis,phylogenetic tree construction and ancestral analysis were carried out with Tibetan sheep,Mongolian sheep and Yunnan sheep,and the unique molecular markers of Awang sheep were screened.The results showed that Awang sheep is of Tibetan line,which is quite different from Mongolian sheep and Yunnan sheep,and it is also a unique group in Tibetan sheep.Genome wide association analysis showed that the black/brown trait of coat color in Awang sheep is significantly correlated with five SNPs on chromosome 14 and chromosome 20.These SNPs are adjacent to or located in two known genes MC1R and TCF25.By comparing with the genome of large samples of sheep at home and abroad,8 breed specific SNPs of Awang sheep were screened for breed identification.To sum up,Awang sheep has genomic specificity and is a unique genetic resource of Tibetan sheep.

关 键 词：阿旺绵羊 群体遗传结构 芯片 藏系绵羊 

分 类 号：S826.2[农业科学—畜牧学]