豇豆抗锈病全基因组关联分析  

Genome Wide Association Study of Rust Resistance in Cowpea

作　　者：吴健[1,2] 汪宝根 陈小央[3] 吴晓花 李潇[1,2] 韩磊 汪颖 王尖 鲁忠富 杨易[4] 张艳 李国景[2] 吴新义[2,5] WU Jian;WANG Baogen;CHEN Xiaoyang;WU Xiaohua;LI Xiao;HAN Lei;WANG Ying;WANG Jian;LU Zhongfu;YANG Yi;ZHANG Yan;LI Guojing;WU Xinyi(College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300;Institute of Vegetable,Zhejiang Academy of Agricultural Sciences/Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Hangzhou 310021;General Station for Seed Administration of Zhejiang Province,Hangzhou 310020;Institute of Vegetable,Guangdong Academy of Agricultural Sciences,Guangzhou 510640;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021)

机构地区：[1]浙江农林大学园艺科学学院,杭州311300 [2]浙江省农业科学院蔬菜研究所/农业农村部南方豆类蔬菜种质创新与分子育种重点实验室(部省共建),杭州310021 [3]浙江省种子管理总站,杭州310020 [4]广东省农业科学院蔬菜研究所,广州510640 [5]浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室,杭州310021

出　　处：《植物遗传资源学报》2024年第11期1907-1922,共16页Journal of Plant Genetic Resources

基　　金：2022-2024年浙江省农作物种质资源精准鉴定评价任务(2023R23T60D01,2022R23T60D04);国家自然科学基金(32172568);浙江省农业新品种选育重大科技专项(2021C02065-6-3);山东省重点研发计划(2023LZGCQY012)。

摘　　要：豇豆是世界范围内重要的豆类作物,在我国既是重要的杂粮作物,也是传统的豆类蔬菜作物。锈病是豇豆生产上三大主要病害之一,选育抗锈病品种是防治锈病最经济有效的策略,筛选抗锈病种质、解析其遗传基础是抗锈病育种的关键和前提。本研究对215份豇豆地方品种进行抗锈病精准鉴定,筛选出40份抗锈病种质。利用重测序技术鉴定了215份种质的基因型,获得3880169个高质量SNPs和469398个高质量InDels。群体结构分析将之分为4个亚群,亚群分类与荚长、荚型高度相关。采用全基因组关联分析,鉴定出10个与锈病抗性显著相关的基因组区段,分布在7条染色体上。单倍型分析显示各峰值SNP的不同等位变异与锈病抗性高度相关,且随着有利等位变异的聚合,群体内抗病材料的比例显著升高。本研究鉴定出10个可能的抗锈病候选基因,包括含BTB/POZ结构域蛋白基因、LRR受体类丝氨酸/苏氨酸激酶基因、乙烯反应转录因子、交替氧化酶基因等。本研究为豇豆抗锈病分子育种提供了理论指导和基因资源。Cowpea is one of the most important legume crops worldwide,serving not only as an important coarse cereal but also as a traditional vegetable in China.Rust is one of the three major diseases in cowpea,and developing rust resistance cultivars is the most cost-effective strategy in disease control.Exploring the resistant germplasm and elucidating the genetic basis of rust resistance is the key and prerequisite for cowpea rust resistance breeding.In the current study,215 cowpea landraces were examined for rust resistance and 40 accessions showing resistance to rust were identified.Through resequencing the 215 accessions,3880169 highquality SNPs and 469398 high-quality InDels were found.These accessions were divided into four subgroups through population structural analysis,and the subgroup division was highly related to pod length and pod type.Total of 10 genomic regions,which significantly associated with rust resistance and distributed on seven chromosomes,were detected by GWAS.Haplotype analysis based on the associated SNPs showed that their alleles were highly correlated to rust resistance.The ratio of resistance accessions increased significantly,when pyramiding increasing number of favored alleles.Ten candidate genes were identified,including BTB/POZ domain-containing protein,LRR receptor-like serine/threonine-protein kinase,ethylene-responsive transcription factor and alternative oxidase genes.These results will provide the theoretical guidance and gene resources for molecular breeding of rust resistance in cowpea.

关 键 词：豇豆 锈病 关联分析 重测序 

分 类 号：S643.4[农业科学—蔬菜学]