印度洋深海来源真菌Penicillium sp.SCSIO 06871次级代谢产物研究  

Study on the secondary metabolites from Penicillium sp.SCSIO 06871 of the deep-sea Indian Ocean

作　　者：庞小艳[1,2] 李晓琳 王俊锋 田新朋[1,2] 徐石海 刘永宏[1,2] PANG Xiaoyan;LI Xiaolin;WANG Junfeng;TIAN Xinpeng;XU Shihai;LIU Yonghong(Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Chemistry and Materials Science,Jinan University,Guangzhou 510632,China)

机构地区：[1]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东广州510301 [2]中国科学院大学,北京100049 [3]暨南大学化学与材料学院,广东广州510632

出　　处：《热带海洋学报》2024年第6期190-195,共6页Journal of Tropical Oceanography

基　　金：广州市科技计划项目(202201010678);海南省科技计划三亚崖州湾科技城联合项目(2021JJLH0097);国家自然科学基金项目(42006084)。

摘　　要：本文对印度洋深海来源真菌Penicillium sp.SCSIO 06871的次级代谢产物进行了研究。采用硅胶、Sephadex LH-20凝胶、和反相ODS (octadecylsilyl)柱色谱、及半制备高效液相色谱法(high performance liquid chromatography,HPLC)分离等手段对该菌株的大米固体培养基发酵产物进行分离纯化,结合核磁共振、质谱、旋光等谱学方法对分离得到的化合物进行结构鉴定,共鉴定7个聚酮类化合物(1—7)和1个吲哚生物碱类化合物(8),结构分别为:sydowinin B(1)、mrlactone1(2)、AGIB4(3)、methylcordyolC(4)、diorcinol(5)、11-dehydrosydonicacid(6)、(+)-sydonicacid(7)和indole-3-acetate(8)。抑菌活性实验显示,化合物4,5和8对多株致病细菌均显示出不同程度的抑制活性。同时α-葡萄糖苷酶抑制活性实验显示,化合物5—8均有较强的α-葡萄糖苷酶抑制活性。The rice fermentation products of the fungus Penicillium sp.SCSIO 06871 from the deep-sea Indian Ocean were studied in this paper.The extract was isolated and purified by silica,Sephadex LH-20,and reversed phase octadecylsilyl(ODS) column chromatography,as well as semipreparative high performance liquid chromatography(HPLC).Eight known compounds,including sydowinin B(1),mrlactone1(2),AGI-B4(3),methyl cordyol C(4),diorcinol(5),11-dehydrosydonic acid(6),(+)-sydonic acid(7),and methyl indole-3-acetate(8) were obtained and identified by analyzing their nuclear magnetic resonance(NMR) spectra,mass spectrum(MS) spectra and specific rotation values.Compounds 4,5 and 8 showed different degree of inhibitory activities against multiple pathogenic bacteria.Also,compounds 5-8 all showed inhibitory activities against α-glycosidase.

关 键 词：深海沉积物来源真菌 Penicilliumsp. 次级代谢产物 抗菌 α-葡萄糖苷酶抑制活性 

分 类 号：Q936[生物学—微生物学]