海绵来源真菌Aspergillus sp.SCSIO 41018的次级代谢产物及其自由基清除活性研究  

Secondary metabolites from the sponge-derived fungus Aspergillus sp.SCSIO 41018 and their free radical scavenging activity

作　　者：袁媛 张媛媛 许福泉 刘庆超[1] 王俊锋[2,4] YUAN Yuan;ZHANG Yuanyuan;XU Fuquan;LIU Qingchao;WANG Junfeng(School of Chemical Engineering,Northwest University,Xi’an 710069,China;Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Sanya Institute of Ocean Eco-Environmental Engineering,Sanya 572000,China)

机构地区：[1]西北大学化工学院,陕西西安710069 [2]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,广东广州510301 [3]江苏海洋大学海洋科学与水产学院,江苏连云港222005 [4]三亚海洋生态环境工程研究院,海南三亚572000

出　　处：《热带海洋学报》2024年第6期196-200,共5页Journal of Tropical Oceanography

基　　金：海南省科技计划三亚崖州湾科技城联合项目(2021JJLH0097);广东省区域联合基金-重点项目(2022B1515120075)。

摘　　要：本文所研究的曲霉属真菌(Aspergillus sp.SCSIO 41018)分离自广东省徐闻县的美丽海绵属海绵(Callyspongia sp.)。通过硅胶柱色谱,Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等分离方法对其大米发酵物进行分离纯化,利用核磁共振波谱和质谱等测试技术,参考已报道的化合物数据,对得到的7个次生代谢产物进行结构鉴定,分别为:cycloechinulin(1)、4-methyl-1H-pyrazole-3-carboxylicacid(2)、diorcinol(3)、methyl-4-hydroxy-2-(3-hydroxy-5-methylphenoxy)-6-methylbenzoate(4)、3,4-二羟基苯乙酸(5)、3,4-二羟基苯甲酸(6)和吲哚-3-甲醛(7),其中化合物2为首次从曲霉属真菌中分离获得。对分离到的化合物1—6进行自由基清除活性试验,结果表明化合物5和6的自由基清除能力均强于阳性对照抗坏血酸(vitaminC,Vc),是强效的抗氧化剂。This paper reported the identification of seven compounds from the rice ferment of Aspergillus sp.fungus SCSIO 41018isolated from sponge Callyspongia sp.in the Xuwen County,Guangdong Province,China.These compounds were purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and semi-preparative high performance liquid chromatography.The seven compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry to determine their structures,in combination with the reported data,as:cycloechinulin(1),4-methyl-1H-pyrazole-3-carboxylic acid(2),diorcinol(3),methyl-4-hydroxy-2-(3-hydroxy-5-methylphenoxy)-6-methylbenzoate(4),benzeneacetic acid(5),protocatechuic acid(6),indole-3-carboxaldehyde(7),wherein compound 2 was first isolated from Aspergillus sp.fungi.The free radical scavenging activity was evaluated,and the results showed that compounds 5 and 6 had stronger free radical scavenging ability and antioxidants potential than the positive control ascorbic acid(Vc,vitamin C).

关 键 词：Aspergillussp. 真菌 次级代谢产物 自由基清除活性 

分 类 号：Q936[生物学—微生物学]