不同激素对红丝青叶组培苗生长及生理的影响  

The Effect of Different Hormones on Growth and Physiology of Hygrophila polysperma Tissue Culture Seedlings

作　　者：郭雅宁 赵震扬 梁梦婷 王梅 郭智勇 管连庆 马雯雯 姜炳阳 刘明慧 李俞静 孙验玲 GUO Yaning;ZHAO Zhenyang;LIANG Mengting;WANG Mei;GUO Zhiyong;GUAN Lianqing;MA Wenwen;JIANG Bingyang;LIU Minghui;LI Yujing;SUN Yanling(School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao Shandong 266237,China)

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266237

出　　处：《种子》2024年第11期42-48,共7页Seed

基　　金：国家自然科学基金青年基金项目(31902408);山东省自然科学基金面上项目(ZR2023MC154);厦门市智慧渔业重点实验室开放课题(XMKLIF-OP-202406);校级实验技术研究课题(SYJS202326);校级课程思政教育专项(XJY2022141)

摘　　要：以水草红丝青叶组培苗为试验材料,研究不同浓度生长素(IAA、IBA、2,4-D)和6-KT在组培培养基中单独或联合使用对其生长及生理应答的变化影响。结果表明,在0.5~3.0μg/μL范围内,单独使用IBA或2,4-D的组培苗表型优于单独使用IAA或6-KT。当IAA或6-KT浓度等于或大于2.0μg/μL时,组培苗发育迟缓;在1.0μg/μL IBA和1.0μg/μL 6-KT或0.5μg/μL IBA和2.0μg/μL 6-KT联合使用早期,组培苗长势均突出,叶片的丙二醛含量较低,叶绿素、可溶性蛋白和脯氨酸含量较高。1.0~2.0μg/μL IBA和2.0μg/μL 6-KT联合使用早期抑制组培苗生长,长期使用会导致其茎尖烧灼。The tissue culture seedling of water plant Hygrophila polysperma was taken as the experimental material,the effects of different concentrations of auxin(IAA,IBA,2,4-D)and 6-KT on the growth and physiological response were studied in tissue culture medium.The results showed that the phenotype of tissue culture seedlings using IBA or 2,4-D alone was superior to IAA or 6-KT in the range of 0.5-3.0μg/μL.When IAA or 6-KT concentration was equal to or greater than 2.0μg/μL,the growth of tissue culture seedlings was delayed.At the early stage of combined use of 1.0μg/μL IBA and 1.0μg/μL 6-KT or 0.5μg/μL IBA and 2.0μg/μL 6-KT,the growth of tissue culture seedlings was prominent,and the contents of malondialdehyde in leaves were lower,and the contents of chlorophyll,soluble protein and proline were higher.The combination of 1.0-2.0μg/μL IBA and 2.0μg/μL 6-KT inhibited the growth of tissue culture seedlings in the early stage,and led to the burning of the stem tips in the long term.

关 键 词：红丝青叶 组织培养 IBA 6-KT 

分 类 号：S682.32[农业科学—观赏园艺]