一株犬小孢子菌拮抗内生芽孢杆菌Y16的分离鉴定  

Isolation and Identification of Antagonistic Endophytic Bacillus Y16 on Microsporum Canis

作　　者：汤兴丽[1] 台立 杨成[1] TANG Xing-li;TAI Li;YANG Cheng(Department of Microbiology and Immunology,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院微生物学与免疫学教研室,安徽芜湖241002

出　　处：《安徽化工》2024年第5期48-51,共4页Anhui Chemical Industry

基　　金：皖南医学院中青年科研基金(WK202120,WK202221)。

摘　　要：从车前草组织中分离到一株对犬小孢子菌有较强拮抗性的内生芽孢杆菌Y16,结合形态特征、生理生化特征及16SrDNA序列分析对该菌株进行鉴定,通过牛津杯法对该菌株发酵液抗菌活性进行复筛及抗菌谱的测定。结果表明,根据形态特征、生理生化特征及16SrDNA序列比对,该菌株被初步鉴定为解淀粉芽孢杆菌。复筛结果表明,该菌株发酵液对犬小孢子菌、白色念珠菌、黑曲霉及革兰氏阳性细菌具有抑制活性。An endophytic bacillus strain with strong inhibitory activity against Microsporum canis was isolated from the tissue of plantago plantifolia.The strain was classified and identified by morphological characteristics,physiological and biochemical characteristics and 16SrDNA sequence analysis.The antibacterial activity and antibacterial spectrum of the fermentation broth were determined by oxford cup method.The results showed that the strain was preliminarily identified as Bacillus amyloliticus based on its morphological characteristics,physiological and biochemical characteristics and 16SrDNA sequence alignment.The fermentation broth of the strain Y16 had inhibitory activity against Microsporum ca⁃nis,Candida albicans,Aspergillus Niger and gram-positive bacteria.

关 键 词：犬小孢子菌 车前草 内生芽孢杆菌 鉴定 抗菌活性 

分 类 号：S476.9[农业科学—农业昆虫与害虫防治]