长链非编码RNA HOTAIR、RAB27A在慢性阻塞性肺疾病病人中的表达及其临床意义  

Expression and clinical significance of long non-coding RNA HOTAIR and RAB27A in COPD patients

作　　者：唐咏婕 李材忠 黎海翔 蔡潇阳 王秀清 TANG Yongjie;LI Caizhong;LI Haixiang;CAI Xiaoyang;WANG Xiuqing(Department of Respiratory,Danzhou People's Hospital,Danzhou,Hainan 571799,China)

机构地区：[1]儋州市人民医院呼吸内科,海南儋州571799

出　　处：《安徽医药》2024年第12期2428-2433,共6页Anhui Medical and Pharmaceutical Journal

基　　金：海南省医药卫生科研基金项目(21A200151)。

摘　　要：目的探讨长链非编码RNA(lncRNA)HOTAIR及lncRNA RAB27A与慢性阻塞性肺疾病(COPD)严重程度的关系和预测COPD急性加重期(AECOPD)死亡的价值。方法选取2020年1月至2022年12月儋州市人民医院收治的COPD病人205例和体检正常者50例(健康对照组),检测所有研究对象血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)、lncRNA HOTAIR及RAB27A水平,记录肺功能指标。根据COPD病人急性加重情况分为稳定期COPD组(98例)和AECOPD组(107例),根据GOLD分级标准将COPD病人分为轻度48例、中度74例,重度63例和极重度20例。根据107例AECOPD病人住院28 d内发生死亡情况,将其分为存活组(63例)和死亡组(44例)。应用多因素logistic回归分析影响AECOPD病人死亡的危险因素,以受试者操作特征曲线(ROC曲线)分析lncRNA HOTAIR及RAB27A预测AECOPD病人死亡的价值。采用Pearson相关分析检验AECOPD病人lncRNA HOTAIR及RAB27A表达水平与炎症因子及肺功能指标的相关性。结果AECOPD组血清lncRNA HOTAIR(3.18±1.45比1.96±0.91,1.13±0.24)及RAB27A(1.90±0.84比1.07±0.15,0.46±0.08)表达水平明显高于稳定期COPD组和健康对照组(P<0.05),且稳定期COPD组高于健康对照组(P<0.05)。极重度组和重度组血清lncRNA HOTAIR(4.10±1.72,2.97±1.34比2.05±0.96,1.82±0.75)及RAB27A(2.61±1.05,1.73±0.62比1.12±0.18,0.86±0.10)表达水平均明显高于轻度组和中度组(P<0.05),且极重度组高于重度组(P<0.05)。死亡组血清lncRNA HOTAIR(3.80±1.63比2.61±1.20)及RAB27A(2.36±0.98比1.53±0.47)表达水平明显高于存活组(P<0.05)。多因素logistic回归分析显示,血清lncRNA HOTAIR[OR=3.38,95%CI:(2.49,10.11)]及lncRNA RAB27A[OR=2.90,95%CI:(2.22,9.42)]表达水平升高是影响AECOPD病人死亡的危险因素(P<0.05)。ROC曲线分析显示,lncRNA HOTAIR≥3.28及RAB27A≥2.01联合预测AECOPD病人死亡的曲线下面积(AUC)高达0.94[95%CI:(0.88,0.99)],灵敏度为97.2%,特异度为84.8%。相关性分析显示,AECOPD病Objective To investigate the relationship between long-chain non-coding RNA HOTAIR(lncRNA HOTAIR)and longchain non-coding RNA RAB27A(lncRNA RAB27A)and the severity of chronic obstructive pulmonary disease(COPD)and their value in predicting the death of acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods Totally 205 patients with COPD and 50 patients with normal physical examination(control group)admitted to Danzhou People's Hospital from January 2020 to December 2022 were selected.The levels of serum interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),lncRNA HOTAIR and RAB27A were detected in all subjects,and lung function indexes were recorded.According to the acute exacerbation of COPD,the patients were assigned into stable COPD group(n=98)and AECOPD group(n=107).According to the GOLD grading standards,the patients were assigned into mild 48 cases,moderate 74 cases,severe 63 cases and extremely severe 20 cases.According to the deaths of 107 AECOPD patients within 28 days of hospitalization,they were assigned into a survival group(n=63)and a death group(n=44).Multivariate logistic regression was used to analyze the risk factors affecting the death of patients with AECOPD.Receiver operating characteristic curve(ROC)were drawn to analyze the value of lncRNA HOTAIR and RAB27A in predicting the death of patients with AECOPD.Pearson correlation was used to analyze the correlation between the expression levels of lncRNA HOTAIR and RAB27A and the inflammatory factors and lung function indicators in AECOPD patients.Results The expression levels of serum lncRNA HOTAIR(3.18±1.45 vs.1.96±0.91,1.13±0.24)and RAB27A(1.90±0.84 vs.1.07±0.15,0.46±0.08)in AECOPD group were significantly higher than those in stable COPD group and control group(P<0.05),and the expression levels in stable COPD group was higher than that in control group(P<0.05).The expression levels of serum lncRNA HOTAIR(4.10±1.72,2.97±1.34 vs.2.05±0.96,1.82±0.75)and RAB27A(2.61±1.05,1.73±0.62 vs.1.12±0.

关 键 词：肺疾病 慢性阻塞性 长链非编码RNA HOTAIR 长链非编码RNA RAB27A 疾病严重程度 预后 

分 类 号：R563.9[医药卫生—呼吸系统]