橄榄苦苷调节JAK2/STAT3信号通路对胆管癌细胞恶性生物学行为的影响  

Impacts of Oleuropein on the Malignant Biological Behavior of Cholangiocarcinoma Cells by Regulating the JAK2/STAT3 Signaling Pathway

作　　者：郑伟 韩朝 ZHENG Wei;HAN Chao(Shaanxi Nuclear Industry 215 Hospital,Shaanxi Xianyang 712000,China)

机构地区：[1]陕西省核工业二一五医院普外二科,陕西咸阳712000

出　　处：《河北医学》2024年第11期1825-1830,共6页Hebei Medicine

基　　金：陕西省2023年院级科研课题立项项目,(编号:215KYJJ-202316)。

摘　　要：目的:探讨橄榄苦苷(Ole)调节Janus激酶2(JAK2)/信号转导和转录激活因子3(STAT3)信号通路对胆管癌细胞恶性生物学行为的影响。方法:将对数生长期HuCCT1细胞分为control组、100μg/mL Ole组、200μg/mL Ole组、400μg/mL Ole组、800μg/mL Ole组和Ole+JAK2/STAT3信号通路激活剂(Colivelin)组,control组不做处理,100μg/mL Ole组、200μg/mL Ole组、400μg/mL Ole组、800μg/mL Ole组分别以100、200、400、800μg/mL的Ole干预HuCCT1细胞,Ole+Colivelin组(分别以800μg/mL Ole和0.5μmoL/L Colivelin干预HuCCT1细胞)。CCK-8法、平板克隆法、流式细胞术分别测定细胞活力、克隆及凋亡情况;Transwell实验检测细胞迁移和侵袭;Western blot法测定JAK2/STAT3信号通路蛋白及凋亡蛋白表达。结果:100、200、400、800μg/mL Ole组HuCCT1细胞活力及克隆细胞数均低于control组,凋亡率高于control组,且变化趋势均呈Ole剂量依赖性(P<0.05);与800μg/mL Ole组对比,Ole+Colivelin组HuCCT1细胞活力及克隆细胞数均升高,凋亡率降低(P<0.05)。与control组对比,100、200、400、800μg/mL Ole组HuCCT1细胞迁移及侵袭数均呈Ole剂量依赖性降低(P<0.05);与800μg/mL Ole组对比,Ole+Colivelin组HuCCT1细胞迁移及侵袭数均升高(P<0.05)。与control组对比,100、200、400、800μg/mL Ole组HuCCT1细胞p-JAK2/JAK2、p-STAT3/STAT3、Bcl-2蛋白表达均呈Ole剂量依赖性降低,Bax蛋白表达呈Ole剂量依赖性升高(P<0.05);与800μg/mL Ole组对比,Ole+Colivelin组HuCCT1细胞p-JAK2/JAK2、p-STAT3/STAT3、Bcl-2蛋白表达均升高,Bax蛋白表达降低(P<0.05)。结论:Ole可能通过抑制JAK2/STAT3信号通路活化进而抑制胆管癌细胞恶性生物学行为。Objective:To investigate the impacts of Oleuropein(Ole)on the malignant biological behavior of cholangiocarcinoma cells by regulating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:HuCCT1 cells in logarithmic growth phase were divided into control group,100μg/mL Ole group,200μg/mL Ole group,400μg/mL Ole group,800μg/mL Ole group,and Ole+JAK2/STAT3 signaling pathway activator(Colivelin)group.The control group was not treated,and HuCCT1 cells in 100μg/mL Ole group,200μg/mL Ole group,400μg/mL Ole group,and 800μg/mL Ole groups were treated with 100,200,400,and 800μg/mL Ole,and the Ole+Colivelin group treated HuCCT1 cells with(800μg/mL Ole and 0.5μmoL/L Colivelin respectively).CCK-8 method,plate cloning method,and flow cytometry were applied to determine cell viability,cloning,and apoptosis,respectively.Transwell experiment was applied to detect cell migration and invasion.Western blot was applied to determine the expression of JAK2/STAT3 signaling pathway proteins and apoptotic proteins.Results:The HuCCT1 cell viability and number of cloned cells in the 100,200,400,and 800μg/mL Ole groups were lower than those in the control group,and the apoptosis rate was higher than that in the control group,the trend of change was Ole dose-dependent(P<0.05).Compared with the 800μg/mL Ole group,the HuCCT1 cell viability and number of cloned cells in the Ole+Colivelin group increased,while the apoptosis rate decreased(P<0.05).Compared with the control group,the migration and invasion of HuCCT1 cells in the 100,200,400,and 800μg/mL Ole groups showed an Ole dose-dependent decrease(P<0.05).Compared with the 800μg/mL Ole group,the migration and invasion of HuCCT1 cells in the Ole+Colivelin group increased(P<0.05).Compared with the control group,the protein expression of p-JAK2/JAK2,p-STAT3/STAT3,and Bcl-2 in HuCCT1 cells in the 100,200,400,and 800μg/mL Ole groups showed an Ole dose-dependent decrease,the protein expression of Bax showed an Ole dose-dependent in

关 键 词：橄榄苦苷 Janus激酶2/信号转导和转录激活因子3 胆管癌 恶性生物学行为 

分 类 号：R285[医药卫生—中药学]