Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures  

作　　者：Joshua Weygant Ali Entezari Fritz Koch Ricardo AndréGalaviz Carlos Ezio Garciamendez Pável Hernández Vanessa Ortiz David Sebastián Rendon Ruiz Francisco Aguilar Andrea Andolfi Ling Cai Sushila Maharjan Anayancy Osorio Yu Shrike Zhang 

机构地区：[1]Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Cambridge,Massachusetts,USA [2]School of Biomedical Engineering,University of Technology Sydney,Broadway,New South Wales,Australia [3]Laboratory for Bioinspired Materials for Biomedical Engineering,IMTEK—Department of Microsystems Engineering,University of Freiburg,Freiburg,Germany [4]Department of Informatics,Bioengineering,Robotics and Systems Engineering(DIBRIS),University of Genova,Genova,Italy [5]Department of Engineering,University of Cambridge,CB21PZ Cambridge,UK

出　　处：《Aggregate》2024年第5期433-444,共12页聚集体（英文）

基　　金：Chan Zuckerberg Initiative,Grant/Award Number:2022-316712;National Science Foundation,Grant/Award Numbers:CBET-EBMS-1936105,CISE-IIS-2225698;National Institutes of Technology,Grant/Award Numbers:R56EB034702,R01CA282451;Brigham Research Institute;National Institutes of Health,Grant/Award Numbers:R01CA282451,R56EB034702。

摘　　要：Droplet-based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine.However,it requires bioinks with low viscosities,which makes it challenging to create complex 3D structures and spatially pattern them with different materials.This study introduces a novel approach to bioprinting sophisti-cated volumetric objects by merging droplet-based bioprinting and cryobioprinting techniques.By leveraging the benefits of cryopreservation,we fabricated,for thefirst time,intricate,self-supporting cell-free or cell-laden structures with single or multiple materials in a simple droplet-based bioprinting process that is facilitated by depositing the droplets onto a cryoplate followed by crosslinking during revival.The feasibility of this approach is demonstrated by bioprinting several cell types,with cell viability increasing to 80%–90%after up to 2 or 3 weeks of culture.Furthermore,the applicational capabilities of this approach are showcased by bio-printing an endothelialized breast cancer model.The results indicate that merging droplet and cryogenic bioprinting complements current droplet-based bioprinting techniques and opens new avenues for the fabrication of volumetric objects with enhanced complexity and functionality,presenting exciting potential for biomedical applications.

关 键 词：3D bioprinting BIOFABRICATION cryogenic bioprinting droplet printing INKJET tissue engineering 

分 类 号：R318[医药卫生—生物医学工程]