A simple“spraying”fluorescence-guided surgery by AIE probes for liver tumor resection through configuration-induced cross-identification  

作　　者：Didi Chen Tian Xiao Liangjie Wang Sijie Chen Chuen Kam Guoping Zeng Li Peng Jinxiang Zhang Min Li Yuping Dong 

机构地区：[1]Hubei Key Laboratory of Purification and Application of Plant Anti-Cancer Active Ingredients,Hubei University of Education,Wuhan,China [2]Department of Emergency Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China [3]School of Life Sciences,The Chinese University of Hong Kong,Hong Kong,China [4]Department of Pathology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China [5]Department of Hepatobiliary Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China [6]Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications,School of Materials Science and Engineering,Beijing Institute of Technology,Beijing,China

出　　处：《Aggregate》2024年第4期294-307,共14页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82172754,81874208;Opening Project of Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients,Grant/Award Number:HLPAI2023001。

摘　　要：Surgical resection is the preferred option for hepatocellular carcinoma(HCC),but surgical navigation technology using indocyanine green still has some drawbacks such as non-specific imaging,thus it is very important to develop newfluorescence imaging technology.All-cis hexaphenyl-1,3-butadiene derivative(ZZ-HPB-NC)with aggregation-induced emission(AIE)feature has been reported to be quickly turned-onfluorescent response in the intraoperative frozen-section slides of HCC.However,the probe did not respond to normal liver tissue around HCC.In order to enhance the diagnostic rate and elucidate the response mechanism,all-trans config-uration EE-HPB-NC,was furtherly synthesized.Within two minutes,non-cancer tissues could befluorescently labeled by EE-HPB-NC by spraying,showing the same effect with ZZ-HPB-NC to HCC.The results indicated that the configuration-induced cross-identificationfluorescence imaging strategy was achieved through the combination of ZZ-and EE-HPB-NC.Then the mechanism of HPB-NC localization in HCC lesions was explored,and the binding of HPB-NC with specific proteins in cells resulted in the AIE effect to label HCC cells.On this basis,the accuracy of specificfluorescence imaging for HCC was further verified on the mouse hepatic neoplasm models,indicating that it has clinical application potential for surgicalfluorescence real-time navigation.

关 键 词：aggregation-induced emission cis–trans isomerism configuration-induced cross-identification fluorescence-guided surgery liver tumor 

分 类 号：O657.3[理学—分析化学] R735.7[理学—化学]