Ultrabright NIR AIEgen nanoparticles-enhanced lateral flow immunoassay platform for accurate diagnostics of complex samples  

作　　者：Jia Shu Yujian Li Huan Cai Qing Fu Chunyang Li Jianbo Yuan Yan Zhao Changjin Liu Haiping Wu Doudou Ling Zhangluxi Liu Guannan Su Qingfeng Cao Xiaolin Huang Rui Chen Peizeng Yang 

机构地区：[1]The First Affiliated Hospital of Chongqing Medical University,Chongqing Branch(Municipality Division)of National Clinical Research Center for Ocular Diseases,Chongqing,China [2]Department of Orthopedics,The First Affiliated Hospital of Chongqing Medical University,Orthopaedic Research Laboratory,Chongqing Medical University,Chongqing,China [3]Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education),College of Laboratory Medicine,Chongqing Medical University,Chongqing,China [4]State Key Laboratory of Food Science and Resources,School of Food Science and Technology,Nanchang University,Nanchang,China

出　　处：《Aggregate》2024年第4期308-318,共11页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82202642,82302646,32172296;Chongqing Postdoctoral Innovation Talent Support Program,Grant/Award Number:CQBX202218;China Postdoctoral Science Foundation,Grant/Award Numbers:2022M710558,2023T160771;Special foundation of Chongqing Postdoctoral Research Programme,Grant/Award Number:2021XM2036;Talent Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education)of College of Laboratory Medicine,Grant/Award Number:JYPY202202;Chongqing Postdoctoral Science Foundation,Grant/Award Number:CSTB2022NSCQ-BHX0689;National Natural Science Foundation Key Program,Grant/Award Numbers:82230032,81930023;Key Project of Chongqing Science and Technology Bureau,Grant/Award Number:CSTC2021jscx-gksb-N0010;Chongqing Outstanding Scientists Project(2019);Chongqing Chief Medical Scientist Project(2018);Chongqing Science and Technology Bureau Mountaineering Project,Grant/Award Numbers:cyyy-xkdfjh-jcyj-202301,cyyy-xkdfjh-lcyj-202303,cyyy-xkdfjh-cgzh-202302。

摘　　要：Accurate and sensitive near-infrared(NIR)luminescent lateralflow immunoassay(LFIA)has attracted considerable attention in thefield of point-of-care testing(POCT).However,the detection accuracy and sensitivity are often compromised by the lowfluorescence quantum efficiency of the NIRfluorescent probe.(<10%)Herein,ultrabright NIR AIEgen nanoparticles(PS@AIE830NPs)composed of polystyrene(PS)nanoparticles and NIR aggregation-induced emission luminogen(AIEgen)with the maximum emission at 830 nm(AIE830)is reported,and its poten-tial to promote an accurate and sensitive detection of complex samples by LFIA is described.The relative quantum yield(QY)of the PS@AIE830NPs was 14.76%,which was superior to that of the polymer embedding method and indocyanine green(ICG)-based NIR nanoparticles.The PS@AIE830NPs immunolabeled-LFIA com-bined with laboratory-built NIR-LFIA portable quantitative instruments(detected light range 800 nm)completely eliminated background interference and allowed>highly accurate and sensitive detection without any pre-treatment steps.The limits of detection(LODs)for aflatoxin B1(AFB1)in soy sauce,alpha hemolysin(Hla)of Staphylococcus aureus biomarker in jointfluid,and C-reactive protein(CRP)in human haemolysed samples were 0.01 ng mL^(-1),0.02µg mL^(-1),and 0.156 mg L^(-1),respectively,commensurating with those of the corresponding gold standard assays and covering the detection range of interests.It is anticipated that the ultrabright NIR AIEgen nanoparticles will serve as a universally applicable signal probe for NIR-LFIA diagnostics,promising to expand the range of applications for quantitative detection of complex samples.

关 键 词：aggregation-induced emission complex sample lateralflow immunoassay NEAR-INFRARED rapid diagnostic testing 

分 类 号：TB383[一般工业技术—材料科学与工程]