霍山石斛内生真菌Trichoderma sp.次生代谢产物研究  

Secondary metabolites of endophytic fungus Trichoderma sp.in Dendrobium huoshanense

作　　者：孙露萍 王举涛 程耀堂 SUN Lu-ping;WANG Ju-tao;CHENG Yao-tang(Anhui Hospital of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Hefei 230031;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,Hefei 230012)

机构地区：[1]上海中医药大学附属曙光医院安徽医院,合肥230031 [2]安徽中医药大学药学院,合肥230012 [3]中药饮片制造新技术安徽省重点实验室,合肥230012

出　　处：《中南药学》2024年第11期2914-2918,共5页Central South Pharmacy

基　　金：安徽省2020年度中医药传承创新项目(No.2020zcyb08);安徽省高等学校自然科学基金重点项目(No.KJ2021A0597)。

摘　　要：目的研究霍山石斛内生真菌Trichoderma sp.的次生代谢产物。方法采用正相硅胶、葡聚糖凝胶、反相C-18及制备液相结合二极管阵列检测器等多种色谱技术分离纯化,所获得的化合物经紫外光谱、核磁共振波谱和质谱数据鉴定。结果从霍山石斛内生真菌Trichoderma sp.中定向分离并鉴定了1个新化合物及8个色原酮和蒽醌类化合物,分别为trichphenoid(1),2,3-dialkylchromone(2),5-hydroxy-2,3-dimethyl-7-methoxychromone(3),7-hydroxy-2-(2'-hydroxypropyl)-5-methylchromone(4),2,5-二甲基-7-羟基色酮(5),1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone(6),1-hydroxy-3-hydroxymethyl-9,10-anthraquinone(7),pachybasin(8),大黄酚(9)。结论所有化合物均为首次从霍山石斛内生真菌Trichoderma sp.中分离得到,化合物1为新化合物。Objective To determine the secondary metabolites of endophytic fungus Trichoderma sp.in Dendrobium huoshanense.Methods The compounds were separated and purified by a variety of chromatographic techniques such as silica gel,sephadex,octadecylsilyl and preparation HPLC combined with photo-diode array,and the obtained compounds were identified by UV-visible spectroscopy,nuclear magnetic resonance spectroscopy and mass spectrometry data.Results One new compound and eight compounds were isolated and their structures were identified as trichphenoid(1),2,3-dialkylchromone(2),5-hydroxy-2,3-dimethyl-7-methoxychromone(3),7-hydroxy-2-(2'-hydroxypropyl)-5-methylchromone(4),2,5-dimethyl-7-hydroxychromone(5),1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone(6),1-hydroxy-3-hydroxymethyl-9,10-anthraquinone(7),pachybasin(8),and chrysophanol(9).Conclusion All the compounds have been isolated from endophytic fungus Trichoderma sp.in Dendrobium huoshanense for the first time and the compound 1 is new.

关 键 词：霍山石斛 Trichoderma sp. 内生真菌 HPLC-DAD 色原酮 蒽醌 

分 类 号：R284.1[医药卫生—中药学] R283[医药卫生—中医学]