L-arginine loading porous PEEK promotes percutaneous tissue repairthrough macrophage orchestration  

作　　者：Tong Zhao Xingdan Liu Zhuangzhuang Chu Jing Zhao Dongya Jiang Xiaohua Dong Ziyi Lu Kelvin WKYeung Xuanyong Liu Liping Ouyang 

机构地区：[1]Laboratory of Key Technology and Materials in Minimally Invasive Spine Surgery,Tongren Hospital,Shanghai JiaoTong University School of Medicine,China [2]Hongqiao International Institute of Medicine,Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200336,China [3]State Key Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai,200050,China [4]School of Chemistry and Materials Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,1 Sub-lane Xiangshan,Hangzhou,310024,China [5]Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Traum1,Guangdong Engineering Technology,Research Center for Orthopaedic Trauma Repair,Department of Orthopaedics and Traumatology,The University of Hong Kong Shenzhen Hospital,Shenzhen,China

出　　处：《Bioactive Materials》2024年第10期19-33,共15页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(32371397,32000938,U21A20100);The Fundamental Research Funds for The Central Universities(YG2023ZD29);Shenzhen Science and Technology Funding(JCYJ20210324120009026);Laboratory Open Fund of Key Technology and Materials in Minimally Invasive Spine Surgery(2024JZWC-ZDB03,2024JZWC-YBA04);Talent project of Shanghai Tongren Hospital(TRKYRC-xx02)are acknowledged.

摘　　要：Infection and poor tissue repair are the key causes of percutaneous implantation failure. However, there is a lackof effective strategies to cope with due to its high requirements of sterilization, soft tissue healing, andosseointegration. In this work, L-arginine (L-Arg) was loaded onto a sulfonated polyetheretherketone (PEEK)surface to solve this issue. Under the infection condition, nitric oxide (NO) and reactive oxygen species (ROS) areproduced through catalyzing L-Arg by inducible nitric oxide synthase (iNOS) and thus play a role in bacteriasterilization. Under the tissue repair condition, L-Arg is catalyzed to ornithine by Arginase-1 (Arg-1), whichpromotes the proliferation and collagen secretion of L929 and rBMSCs. Notably, L-Arg loading samples couldpolarize macrophages to M1 and M2 in infection and tissue repair conditions, respectively. The results in vivoshow that the L-Arg loading samples could enhance infected soft tissue sealing and bone regeneration. Insummary, L-Arg loading sulfonated PEEK could polarize macrophage through metabolic reprogramming,providing multi-functions of antibacterial abilities, soft tissue repair, and bone regeneration, which gives a newidea to design percutaneous implantation materials.

关 键 词：POLYETHERETHERKETONE Macrophage orchestration STERILIZATION Tissue repair 

分 类 号：R318[医药卫生—生物医学工程]