Creating a bionic scaffold via light-curing liquid crystal ink to reveal the role of osteoid-like microenvironment in osteogenesis  

作　　者：Kun Liu Lin Li Yizhi Li Yiting Luo Zhaoyu Zhang Wei Wen Shan Ding Yadong Huang Mingxian Liu Changren Zhou Binghong Luo 

机构地区：[1]Biomaterial Research Laboratory,Department of Material Science and Engineering,College of Chemistry and Materials,Jinan University,Guangzhou,510632,PR China [2]Engineering Research Center of Artificial Organs and Materials,Ministry of Education,Guangzhou,510632,PR China [3]Department of Cell Biology&Institute of Biomedicine,College of Life Science and Technology,Guangzhou,510632,PR China

出　　处：《Bioactive Materials》2024年第10期244-260,共17页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(31771047);Guangdong Provincial Natural Science Foundation of China(2022A1515010592and 2023A1515010107);Guangdong Provincial Key Areas R&D Programs(2022B1111080007);the Key Areas Research and Development Program of Guangzhou(202103030003);the Outstanding Innovative Talents Cultivation Funded Programs for Doctoral Students of Jinan University(2023CXB013).

摘　　要：Osteoid plays a crucial role in directing cell behavior and osteogenesis through its unique characteristics,including viscoelasticity and liquid crystal(LC)state.Thus,integrating osteoid-like features into 3D printing scaffolds proves to be a promising approach for personalized bone repair.Despite extensive research on viscoelasticity,the role of LC state in bone repair has been largely overlooked due to the scarcity of suitable LC materials.Moreover,the intricate interplay between LC state and viscoelasticity in osteogenesis remains poorly understood.Here,we developed innovative hydrogel scaffolds with osteoid-like LC state and viscoelasticity using digital light processing with a custom LC ink.By utilizing these LC scaffolds as 3D research models,we discovered that LC state mediates high protein clustering to expose accessible RGD motifs to trigger cell-protein interactions and osteogenic differentiation,while viscoelasticity operates via mechanotransduction pathways.Additionally,our investigation revealed a synergistic effect between LC state and viscoelasticity,amplifying cellprotein interactions and osteogenic mechanotransduction processes.Furthermore,the interesting mechanochromic response observed in the LC hydrogel scaffolds suggests their potential application in mechanosensing.Our findings shed light on the mechanisms and synergistic effects of LC state and viscoelasticity in osteoid on osteogenesis,offering valuable insights for the biomimetic design of bone repair scaffolds.

关 键 词：3D printing Digital light process Liquid crystal hydrogel scaffolds High protein clustering Tunable mechanotransduction Synergistic osteogenesis 

分 类 号：R318[医药卫生—生物医学工程]