Interfacial delivery of carbon monoxide via smart titanium implant coating for enhanced soft tissue integration with switchable antibacterial and immunomodulatory properties  

作　　者：Minghao Zhou Gangfeng Li Jingwei Yu Qian Zhou Kun Wang Jiaxin Kang Tengjiao Wang Peng Li Hongbo Wei 

机构地区：[1]State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture,Department of Oral Implants,School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi,PR China [2]Frontiers Science Center for Flexible Electronics(FSCFE),Institute of Flexible Electronics(IFE)&Institute of Biomedical Materials and Engineering(IBME),Northwestern Polytechnical University(NPU),Xi’an,Shaanxi,PR China [3]School of Flexible Electronics,Henan Institute of Flexible Electronics(HIFE),Henan University,379 Mingli Road,Zhengzhou,450046,PR China [4]Chongqing Innovation Center,Northwestern Polytechnical University,Chongqing,401135,PR China

出　　处：《Bioactive Materials》2024年第10期318-333,共16页生物活性材料（英文）

基　　金：support from the Natural Science Foundation of China(52073224 and 52073230);the Shaanxi Provincial Science Fund for Distinguished Young Scholars(2023-JC-JQ-32);Key Research and Development Program of Shaanxi(2024SFYBXM-438 and 2022SF-165);Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0225).

摘　　要：Soft tissue integration around titanium(Ti)implants is weaker than that around natural teeth,compromising long-term success of Ti implants.Carbon monoxide(CO)possesses distinctive therapeutic properties,rendering it as a highly promising candidate for enhancing STI.However,achieving controlled CO generation at the STI interface remains challenging.Herein,a controlled CO-releasing dual-function coating was constructed on Ti surfaces.Under near-infrared(NIR)irradiation,the designed surface could actively accelerate CO generation for antibiosis against both aerobic and anaerobic bacteria.More importantly,in the absence of NIR,the slow release of CO induces macrophage polarization from pro-inflammatory phenotype towards pro-regenerative phenotype.In a rat implantation model with induced infection,the designed surface effectively controlled the bacterial infection,alleviates accompanying inflammation and modulated immune microenvironment,leading to enhanced STI.Single-cell sequencing revealed that the coating alters the cytokine profile within the soft tissue,thereby influencing cellular functions.Differentially expressed genes in macrophages are highly enriched in the PIK3-Akt pathway.Furthermore,the cellular communication between fibroblasts and macrophages was significantly enhanced through the CXCL12/CXCL14/CXCR4 and CSF1-CSF1R ligand-receptor pair.These findings indicate that our coating showed an appealing prospect for enhancing STI around Ti implants,which would ultimately contribute to the improved long-term success of Ti implants.

关 键 词：Gastrotransmitters Soft tissue integration Immune microenvironment Ti implants ANTIBACTERIAL 

分 类 号：TG174.4[金属学及工艺—金属表面处理]