Wet tissue adhesive polymeric powder hydrogels for skeletal muscle regeneration  

作　　者：Mingyu Lee Daun Seo Junggeon Park Sun Hong Lee Jin Jeon Woochan Kim Jangho Kim Hee Seok Yang Jae Young Lee 

机构地区：[1]School of Materials Science and Engineering,Gwangju Institute of Science and Technology,Gwangju,61005,Republic of Korea [2]Department of Nanobiomedical Science&BK21 FOUR NBM Global Research Center for Regenerative Medicine,Dankook University,Cheonan,31116,Republic of Korea [3]Department of Convergence Biosystems Engineering,Chonnam National University,Gwangju,61186,Republic of Korea [4]Department of Rural and Biosystems Engineering,Chonnam National University,Gwangju,Republic of Korea [5]Interdisciplinary Program in IT-Bio Convergence System,Chonnam National University,Gwangju,61186,Republic of Korea

出　　处：《Bioactive Materials》2024年第10期334-344,共11页生物活性材料（英文）

基　　金：supported by a grant from the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT,and Future Planning(2021M3H4A1A04092882 and 2023R1A2C2002802).

摘　　要：Volumetric muscle loss(VML)frequently results from traumatic incidents and can lead to severe functional disabilities.Hydrogels have been widely employed for VML tissue regeneration,which are unfortunately ineffective because of the lack of intimate contact with injured tissue for structural and mechanical support.Adhesive hydrogels allow for strong tissue connections for wound closure.Nevertheless,conventional adhesive hydrogels exhibit poor tissue adhesion in moist,bleeding wounds due to the hydration layer at the tissue–hydrogel interfaces,resulting in insufficient performance.In this study,we developed a novel,biocompatible,wet tissue adhesive powder hydrogel consisting of dextran-aldehyde(dex-ald)and gelatin for the regeneration of VML.This powder absorbs the interfacial tissue fluid and buffer solution on the tissue,spontaneously forms a hydrogel,and strongly adheres to the tissue via various molecular interactions,including the Schiff base reaction.In particular,the powder composition with a 1:4 ratio of dex-ald to gelatin exhibited optimal characteristics with an appropriate gelation time(258 s),strong tissue adhesion(14.5 kPa),and stability.Dex-ald/gelatin powder hydrogels presented strong adhesion to various organs and excellent hemostasis compared to other wet hydrogels and fibrin glue.A mouse VML injury model revealed that the dex-ald/gelatin powder hydrogel significantly improved muscle regeneration,reduced fibrosis,enhanced vascularization,and decreased inflammation.Consequently,our wet-adhesive powder hydrogel can serve as an effective platform for repairing various tissues,including the heart,muscle,and nerve tissues.

关 键 词：POWDER HYDROGEL Wet-adhesive Skeletal muscle regeneration 

分 类 号：O64[理学—物理化学]