Kneadable dough-type hydrogel transforming from dynamic to rigid network to repair irregular bone defects  

作　　者：Ningtao Wang Jie Chen Yanyang Chen Liang Chen Luhan Bao Zhengmei Huang Xiaoyu Han Jiangkuo Lu Zhengwei Cai Wenguo Cui Zhengwei Huang 

机构地区：[1]Department of Endodontics,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,639 Zhizaoju Road,Shanghai,200011,PR China [2]Department of Orthopaedics,Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,197 Ruijin 2nd Road,Shanghai,200025,PR China [3]Department of Stomatology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,160 Pujian Road,Shanghai,200127,PR China

出　　处：《Bioactive Materials》2024年第10期430-444,共15页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(82071104/32101104/82202663);Science and Technology Commission of Shanghai Municipality(23XD1434200/22Y21901000);Program of Shanghai Academic/Technology Research Leader(22XD1422600);Shanghai Municipal Health Planning Commission(202140127);Shanghai Hospital Development Center(SHDC12022120).

摘　　要：Irregular bone defects,characterized by unpredictable size,shape,and depth,pose a major challenge to clinical treatment.Although various bone grafts are available,none can fully meet the repair needs of the defective area.Here,this study fabricates a dough-type hydrogel(DR-Net),in which the first dynamic network is generated by coordination between thiol groups and silver ions,thereby possessing kneadability to adapt to various irregular bone defects.The second rigid covalent network is formed through photocrosslinking,maintaining the osteogenic space under external forces and achieving a better match with the bone regeneration process.In vitro,an irregular alveolar bone defect is established in the fresh porcine mandible,and the dough-type hydrogel exhibits outstanding shape adaptability,perfectly matching the morphology of the bone defect.After photocuring,the storage modulus of the hydrogel increases 8.6 times,from 3.7 kPa(before irradiation)to 32 kPa(after irradiation).Furthermore,this hydrogel enables effective loading of P24 peptide,which potently accelerates bone repair in Sprague–Dawley(SD)rats with critical calvarial defects.Overall,the dough-type hydrogel with kneadability,space-maintaining capability,and osteogenic activity exhibits exceptional potential for clinical translation in treating irregular bone defects.

关 键 词：Kneadable Dough-type hydrogel Dynamic network Rigid network Irregular bone defect 

分 类 号：O64[理学—物理化学]