Engineering biomimetic silk fibroin hydrogel scaffolds with“organic-inorganic assembly”strategy for rapid bone regeneration  

作　　者：Renjie Liang Rui Li Weidong Mo Xianzhu Zhang Jinchun Ye Chang Xie Wenyue Li Zhi Peng Yuqing Gu Yuxuan Huang Shufang Zhang Xiaozhao Wang Hongwei Ouyang 

机构地区：[1]Department of Sports Medicine of the Second Affiliated Hospital,and Liangzhu Laboratory,Zhejiang University School of Medicine,Hangzhou,China [2]Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou,China [3]Zhejiang University-University of Edinburgh Institute,Zhejiang University School of Medicine,Haining,China [4]China Orthopedic Regenerative Medicine Group(CORMed),Hangzhou,China [5]Department of Orthopedics,The First Affiliated Hospital,Zhejiang University School of Medicine,79 Qingchun Rd,Hangzhou,China

出　　处：《Bioactive Materials》2024年第10期541-556,共16页生物活性材料（英文）

基　　金：supported by the National Key Research and Development Program of China(2023YFB3813000);the National Natural Science Foundation of China(T2121004,92268203,32371411).

摘　　要：Although natural polymers have been widely used in constructing bone scaffolds,it still remains challenging to fabricate natural polymer-derived bone scaffolds with biomimetic mechanical properties as well as outstanding osteogenic properties for large-size and weight-bearing bone defects regeneration.Herein,an“organic-inorganic assembly”strategy is developed to construct silk fibroin(SF)-based bone scaffolds with the aforementioned merits.After secondary structure reshuffling,the 3.3-fold increment ofβ-sheet structures in SF hydrogel resulted in a 100-fold improvement of mineral-assembly efficacy via influencing the ion adsorption process and providing templates for mineral growth.Notably,abundant minerals were deposited within the hydrogel and also on the surface,which indicated entire mineral-assembly,which ensured the biomimetic mechanical properties of the digital light processing 3D printed SF hydrogel scaffolds with haversian-mimicking structure.In vitro experiments proved that the assembly between the mineral and SF results in rapid adhesion and enhanced osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.In vivo experiments further proved that the mineral-assembled SF hydrogel scaffold could significantly enhance integration and bone regeneration at the weight-bearing site within one month.This SF-based“organic-inorganic assembly”strategy sheds light on constructing cell-free,growth factor-free and natural polymer-derived bone scaffolds with biomimetic 3D structure,mechanical properties and excellent osteogenic properties.

关 键 词：Silk fibroin Organic-inorganic assembly MINERALIZATION DLP 3D printing Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]