Optimizing UVA and UVC synergy for effective control of harmful cyanobacterial blooms  

作　　者：Yinjie Zhu Jian Ding Xiaoxiong Wang Xuejian Wang Huansheng Cao Fei Teng Shishi Yao Zhiru Lin Yuelu Jiang Yi Tao 

机构地区：[1]Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,Guangdong,518055,China [2]Key Laboratory of Microorganism Application and Risk Control(MARC)of Shenzhen,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,Guangdong,518055,China [3]Shenzhen Key Laboratory of Ecological Remediation and Carbon Sequestration,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,Guangdong,518055,China [4]Tsinghua University-Kunming Joint Research Center for Dianchi Plateau Lake,Tsinghua University,Beijing,100084,China [5]Institute for Ocean Engineering,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China [6]Division of Natural and Applied Sciences,Duke Kunshan University,Kunshan,Jiangsu,215300,China

出　　处：《Environmental Science and Ecotechnology》2024年第6期215-225,共11页环境科学与生态技术（英文）

基　　金：National Natural Science Foundation of China(52070117);Research Projects from Shenzhen Municipal Science and Technology Innovation Council(WDZC20200819163549002,JCYJ20200109142822787);Guangdong Higher Education Institutions Innovative Research Team of Urban Water Cycle and Ecological Safety,China(2023KCXTD053);Shenzhen Science and Technology Program,China(ZDSYS20220606100806014).

摘　　要：Harmful cyanobacterial blooms(HCBs)pose a global ecological threat.Ultraviolet C(UVC)irradiation at 254 nm is a promising method for controlling cyanobacterial proliferation,but the growth suppression is temporary.Resuscitation remains a challenge with UVC application,necessitating alternative strategies for lethal effects.Here,we show synergistic inhibition of Microcystis aeruginosa using ultraviolet A(UVA)pre-irradiation before UVC.We find that low-dosage UVA pre-irradiation(1.5 J cm^(−2))combined with UVC(0.085 J cm^(−2))reduces 85%more cell densities compared to UVC alone(0.085 J cm^(−2))and triggers mazEF-mediated regulated cell death(RCD),which led to cell lysis,while high-dosage UVA pre-irradiations(7.5 and 14.7 J cm^(−2))increase cell densities by 75-155%.Our oxygen evolution tests and transcriptomic analysis indicate that UVA pre-irradiation damages photosystem I(PSI)and,when combined with UVC-induced PSII damage,synergistically inhibits photosynthesis.However,higher UVA dosages activate the SOS response,facilitating the repair of UVC-induced DNA damage.This study highlights the impact of UVA pre-irradiation on UVC suppression of cyanobacteria and proposes a practical strategy for improved HCBs control.

关 键 词：Cyanobacterial bloom Ultraviolet irradiation Photosynthetic damage DNA damage/repair Regulated cell death 

分 类 号：X55[环境科学与工程—环境工程]