动态光散射法测定纳米颗粒粒度的实验室间比对分析  

Interlaboratory comparison for nanoparticle size analysis by dynamic light scattering

作　　者：邓小娟[1] 胡小侠[1] 杜海燕[1] 王意[1] 丁国生[1] 薛涛[1] DENG Xiaojuan;HU Xiaoxia;DU Haiyan;WANG Yi;DING Guosheng;XUE Tao(Analysis and Testing Center,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学分析测试中心,天津300072

出　　处：《实验技术与管理》2024年第11期79-85,共7页Experimental Technology and Management

基　　金：天津大学实验室建设与管理改革项目(LAB2018-B02,LAB2023-01)。

摘　　要：动态光散射法是测定悬浮液中纳米颗粒平均流体力学直径及其粒度分布的重要手段,广泛应用于材料、化工、生物、医药等领域。由于不同仪器、不同人员等因素影响,不同实验室间的测定结果会有一定差异,因而有必要组织实验室间比对,以考察实验室间的数据可比性,提高实验室检测能力。该文介绍了以2023年高校实验室间比对项目“动态光散射法测定纳米颗粒粒度”的开展情况。项目选用均匀稳定的样品作为比对样品,共有27家实验室共28台仪器参加比对,采用稳健统计技术对数据进行统计分析,通过Z比分数对实验室检测能力进行评价。比对结果表明,初测合格率为92.9%。验证了不同实验室间纳米粒度分析数据的可比性,综合分析了影响结果一致性的因素,查找了存在的问题并给出了相关技术建议。[Objective]Dynamic light scattering is one of the effective methods to determine the average hydrodynamic diameter and particle size distribution of nanoparticles in suspension.It is widely used in various fields,such as materials,chemical engineering,biology,and medicine.Due to the influence of different instruments and personnel,the measurement results between different laboratories may have certain differences.Therefore,it is necessary to organize interlaboratory comparisons to examine the comparability of data between laboratories.This helps each participating laboratory identify deficiencies,analyze differences,and effectively prevent them,continuously improving laboratory detection capabilities.[Methods]Taking the 2023 interlaboratory comparison project“Determination of Nanoparticle Particle Size by Dynamic Light Scattering Method”as an example,the project selected polystyrene nanoparticles as the comparison sample and conducted multiple investigations on the uniformity and stability of the samples.One-way analysis of variance was used to test the particle size of the test results to verify the uniformity of the samples.Then,the t-test method was used to analyze the particle size of the test results to test the stability of the samples.At the same time,considering temperature changes during transportation and daily testing,the short-term stability of the samples was examined under room temperature and high-temperature storage environments.A detailed standard operation procedure has been prepared,including sample information,testing methods,testing criteria,results,and evaluation instructions.In addition,the dispersant has been uniformly prepared to provide a basic guarantee for the comparability of experimental results.The data were analyzed using robust statistical techniques,and the results were evaluated by Z-ratio scores.[Results]The results of the sample uniformity test show that there was no significant difference between the samples,indicating that these samples were uniform.The stability test re

关 键 词：动态光散射 粒度 实验室间比对 Z比分数 

分 类 号：O436.2[机械工程—光学工程]