小鼠囊胚不同孵化结局的基因表达分化模式研究  

Differentiation of Mouse Blastocysts with Differential Hatching Outcomes

作　　者：马梅香 吴俞霖 巴合达西·阿达力 刘亚恒 阿依舒阿克·对山别克 安礼友 MA Meixiang;WU Yulin;BAHEDAXI Adali;LIU Yaheng;AYISHUAKE Duishanbieke;AN Liyou(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,School of Life Science and Technology,Xinjiang University,Urumqi Xinjiang 830017,China)

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830017

出　　处：《新疆大学学报（自然科学版中英文）》2024年第6期665-671,共7页Journal of Xinjiang University(Natural Science Edition in Chinese and English)

基　　金：新疆维吾尔自治区自然科学基金杰出青年科学基金“受精卵与输卵管互作对发育结局的影响”(2022D01E09);大学生创新训练计划“囊胚的孵化命运分子决策及预测”(S202210755076).

摘　　要：目前对哺乳动物受精卵到囊胚发育命运的研究较为清楚,但囊胚孵化到着床前的分化过程还不明确,囊胚有两种孵化结局,即孵化和未孵化,故初步分析了囊胚两种孵化结局的发育差异.通过分析不同孵化结局囊胚的转录组,发现囊胚由扩张到孵化过程中基因表达呈现特异的趋势,1433个基因在孵化过程中表达上调,743个基因表达下调;如果囊胚未能孵化,这部分上调基因的表达调控受到显著抑制.孵化与未孵化囊胚间的差异基因涉及免疫与炎症、细胞分化、凋亡等生化过程,提示这些分子可能与着床过程的母胎识别相关.通过qPCR分析发现,孵化囊胚相较未孵化囊胚,基因Podxl、Lama1、Ptk2b表达上调,Pramel17、Amigo2、Pramel31表达下调,数据与转录组分析结果一致.结果表明:小鼠囊胚在孵化过程中基因表达呈现特定的分化,决定了孵化与否的命运结局,分化也与胚胎着床能力形成相关.Current research has significantly enhanced our understanding of the developmental trajectory of mammalian zygotes as they transition into blastocysts.However,the mechanisms governing the differentiation of blastocysts during the hatching phase leading to pre-implantation remain poorly defined.At the blastocyst stage,embryos can either successfully hatch or fail to do so.This study sought to explore the developmental variations between these two hatching outcomes.Through transcriptome analysis,we uncovered distinct gene expression patterns during the transition from expansion to hatching.Our results indicated that during the hatching process,1433 genes were upregulated,while 743 genes experienced downregulation.Importantly,in instances where blastocysts did not hatch,the regulation of the upregulated genes was significantly suppressed.The differentially expressed genes between hatched and non-hatched blastocysts are involved in critical biochemical pathways,including immune and inflammatory responses,cell differentiation,and apoptosis,et al.,suggesting their potential importance in maternal-fetal recognition and implantation.Furthermore,qPCR analysis corroborated the transcriptome findings,revealing that in hatched blastocysts,genes Podxl,Lama1,and Ptk2b were upregulated,whereas Pramel17,Amigo2,and Pramel31 were downregulated.These results imply that the specific patterns of gene expression in mouse blastocysts during the hatching process are pivotal in determining their hatching fate,and are closely associated with the development of embryonic implantation capability.

关 键 词：小鼠囊胚 孵化结局 基因表达 代谢通路 

分 类 号：Q756[生物学—分子生物学]