典型抗生素和纳米材料复合胁迫对污水生物除磷的影响机制探究  

Typical Antibiotics and Nanomaterial Composite Stress Impact Mechanism on Sewage Biological Phosphorus Removal

作　　者：孙书荃 高川 姬尧[1] 张帆 刘金蕾 SUN Shuquan;GAO Chuan;JI Yao;ZHANG Fan;LIU Jinlei(Yellow River Conservancy Technical Institute,Kaifeng 475004,China;Kaifeng Municipal Water Environmental Pollution Monitoring Engineering Technology Research Center,Kaifeng 475004,China;Henan University of Engineering,Zhengzhou 450000,China)

机构地区：[1]黄河水利职业技术学院,河南开封475004 [2]开封市水环境污染监测工程技术研究中心,河南开封475004 [3]河南工程学院,河南郑州450000

出　　处：《水处理技术》2024年第11期100-106,112,共8页Technology of Water Treatment

基　　金：河南省科技发展计划(222102320424);河南省教育厅重点项目(22B610005)。

摘　　要：针对抗生素和纳米材料复合胁迫对污水强化生物除磷工艺(EBPR)运行效能影响不明确的现状,构筑序批式反应器,在中温(30~35℃)条件下考察了环丙沙星(CIP)和纳米氧化锌(NiO NPs)复合胁迫对EBPR的影响并分析典型周期内物质转化规律、污泥特征及比耗氧速率、活性氧、乳酸脱氢酶释放等揭示相关机制。结果表明,CIP和ZnO NPs复合胁迫严重抑制了EBPR运行效能,COD和磷酸盐去除效率分别下降至62.2%~70.6%和71.6%~76.5%,低于CIP和ZnO NPs单独暴露组。CIP和NiO NPs复合胁迫降低了污泥浓度及有机质占比,并提高了胞外聚合物含量及蛋白质和多糖组分的含量。典型周期研究发现CIP和NiO NPs复合胁迫主要降低好氧期COD消耗、厌氧释磷及好氧吸磷。此外,CIP和NiO NPs复合胁迫降低了内聚物聚羟基脂肪酸酯的合成但促进了糖原质代谢,降低了聚磷微生物活性。微生物活性分析表明复合胁迫能降低比耗氧速率,并促进活性氧及乳酸脱氢酶释放,降低微生物代谢。研究结果为EBPR工艺处理含抑菌剂和纳米材料的废水提供一定的技术参考。In response to the unclear impact of composite stress from antibiotics and nanomaterials on the operational efficiency of enhanced biological phosphorus removal processes(EBPR)in wastewater treatment,a sequencing batch reactor was constructed to investigate the effects of ciprofloxacin(CIP)and nano zinc oxide(NiO NPs)composite stress on EBPR under mesophilic conditions(30°C to 35°C).The study analyzed the transformation rules of substances,sludge characteristics,specific oxygen consumption rate,active oxygen,and lactate dehydrogenase release within a typical cycle to reveal the related mechanisms.The results showed that the composite stress of CIP and ZnO NPs severely inhibited the operational efficiency of EBPR,with the removal efficiencies of COD and phosphate decreasing to 62.2%to 70.6%and 71.6%to 76.5%,respectively,which were lower than those in the groups exposed to CIP and ZnO NPs alone.The composite stress of CIP and NiO NPs reduced the sludge concentration and the proportion of organic matter,while increasing the content of extracellular polymeric substances and the components of protein and polysaccharides.The typical cycle study found that the composite stress of CIP and NiO NPs mainly reduced the consumption of COD during the aerobic phase,phosphorus release during the anaerobic phase,and phosphorus absorption during the aerobic phase.In addition,the composite stress of CIP and NiO NPs decreased the synthesis of polyhydroxyalkanoates by intracellular polymers but promoted the metabolism of glycogen,reducing the activity of polyphosphate-accumulating organisms.Microbial activity analysis indicated that composite stress could reduce the specific oxygen consumption rate and promote the release of active oxygen and lactate dehydrogenase,decreasing microbial metabolism.The research results provide technical references for the EBPR process to treat wastewater containing bacteriostatic agents and nanomaterials.

关 键 词：环丙沙星 纳米氧化镍 生物除磷 微生物 

分 类 号：X703[环境科学与工程—环境工程]