香蕉磷脂酶C基因生物信息学及表达分析  

Bioinformatics and Expression Analysis of Banana Phospholipase C Gene

作　　者：殷菲胧 廖玲燕 刘云芬 孙健 郑培君[3] 李丽 帅良 Yin Feilong;Liao Lingyan;Liu Yunfen;Sun Jian;Zheng Peijun;Li Li;Shuai Liang(Institute of Food Science and Engineering Technology,College of Food and Biological Engineering,Hezhou University,Hezhou,542899;Institute of Agricultural Products Processing,Guangxi Academy of Agricultural Sciences,Nanning,530007;School of New Energy and Energy Conservation and Environmental Protection Engineering,Foshan Vocational and Technical College,Foshan,528131)

机构地区：[1]贺州学院食品与生物工程学院,食品科学与工程技术研究院,贺州542899 [2]广西壮族自治区农业科学院农产品加工研究所,南宁530007 [3]佛山职业技术学院新能源与节能环保工程学院,佛山528131

出　　处：《分子植物育种》2024年第22期7309-7318,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860457);广西自然科学基金项目(2018GXNSFBA281118,2019GXNSFBA245037,2020GXNSFAA297153,2020GXNSFBA297111);广西高等教育本科教学改革工程项目(2021JGZ160);广西高校中青年教师科研基础能力提升项目(2020KY18020,2021KY0705);贺州学院校级科研项目(209200215)共同资助。

摘　　要：为探究香蕉磷脂酶C (phospholipase C, PLC)基因的功能,本研究通过blastp结合结构域筛选的方法,从香蕉基因组搜索鉴定出5个磷脂酶C基因,分别命名为MaPI-PLC2、MaPI-PLC6、MaNPC1、MaNPC2和MaNPC6,并对其进行了生物信息学和组织特异性表达分析。生物信息学分析表明,5个香蕉PLC蛋白氨基酸数在515~593之间,等电点在5.72~8.96之间;5个香蕉PLC蛋白均为不稳定的亲水性蛋白;跨膜结构分析发现,只有MaNPC1有跨膜结构,其他4个香蕉PLC蛋白均无跨膜结构;信号肽预测发现,所有的MaNPC均有信号肽,所有的MaPI-PLC均无信号肽。香蕉果实PLC基因不同组织特异性表达分析结果显示,MaPI-PLC-2、MaNPC-2和MaPI-PLC-6基因均在芽、嫩叶和根中有较高表达,MaNPC6在未成熟的果皮和果肉中高度表达,MaNPC1则在成熟的组织(果皮,果肉,老叶)表达含量远高于未成熟组织(果皮,果肉,芽,嫩叶),表明香蕉磷脂酶C全程参与了香蕉发育、成熟和衰老。本研究可为后期香蕉磷脂酶C功能研究奠定基础。To explore the function of banana phospholipase C(PLC) gene, this study uses the balstp binding domain screening method to identify five phospholipase C genes from the banana genome search, named MaPI-PLC2,MaPI-PLC6, MaNPC1, MaNPC2 and MaNPC6, and conducted bioinformatics and tissue-specific expression analysis for them. Bioinformatics analysis shows that the number of amino acids in the PLC protein of 5 bananas is515~593;the theoretical isoelectric point of 5 banana PLC proteins is 5.72~8.96;the 5 banana PLC proteins are unstable hydrophilic proteins;the banana PLC protein transmembrane structure was found, only MaNPC1 has a transmembrane structure, and the other 4 banana PLC proteins have no transmembrane structure. Signal peptide prediction found that all Ma NPCs have signal peptides, and all MaPI-PLCs have no signal peptides. The results of analysis of PLC genes specific expression in different tissues of banana fruit showed that MaPI-PLC2, MaNPC2 and MaPI-PLC6 genes were all highly expressed in buds, young leaves and roots, and MaNPC6 was expressed in immature peels and it was highly expressed in the pulp, while the expression level of MaNPC1 in mature tissues(skin, pulp, old leaves) was much higher than that of immature tissues(skin, pulp, buds, young leaves), indicating that banana phospholipase C is involved in banana development, maturation and senescence throughout the process. This study can lay the foundation for the later research of banana phospholipase C.

关 键 词：香蕉 磷脂酶C 生物信息学 基因表达 

分 类 号：S668.1[农业科学—果树学]