金银花绿原酸基因的发掘及生物信息学分析  

Identification and Bioinformatics Analysis of Gene for Chlorogenic Acid in Lonicera japonica Thunb.

作　　者：李凤梅 陈林鑫 李建军[2] 张梦 齐玉亭 徐鑫[1] 徐小博 陈磊山[1] Li Fengmei;Chen Linxin;Li Jianjun;Zhang Meng;Qi Yuting;Xu Xin;Xu Xiaobo;Chen Leishan(School of Life Sciences&Basic Medicine,Xinxiang University,Xinxiang,453003;College of Life Sciences,Henan Normal University,Xinxiang,453007)

机构地区：[1]新乡学院生命科学与基础医学学院,新乡453003 [2]河南师范大学生命科学学院,新乡453007

出　　处：《分子植物育种》2024年第22期7389-7399,共11页Molecular Plant Breeding

基　　金：河南省高等学校重点科研项目计划课题(21B180009);新乡市重大科技专项(172107000031)共同资助。

摘　　要：绿原酸(chlorogenic acid, CGA)是金银花(Lonicera japonica Thunb.)重要的功效成分,具有抗菌、抗病毒、防癌、防衰老等作用。本研究参照17个物种中33个绿原酸基因编码蛋白的氨基酸序列,通过NCBIBLAST扫描金银花蛋白组数据库,结合亲缘关系分析和结构域分析,筛选获得5个调控金银花绿原酸候选基因,均为Phenylalanine amononia-lyase,分别命名为LjPAL1、LjPAL2、LjPAL3、LjPAL4、LjPAL5。理化性质分析和亚细胞定位发现,这5个候选基因编码的氨基酸个数为686~714,理论等电点为5.85~6.26,均为稳定的亲水性蛋白,均定位在细胞质中。本研究为金银花的分子标记辅助育种提供基因资源,为金银花绿原酸合成代谢途径的研究提供科学依据。C hlorogenic acid is an import ant functional component of Lonicera japonica Thunb., which remain effects of antibacterial, antiretroviral, anti-cancer and anti-aging. In this study, we identified five candidate genes of Phenylalanine amononia-lyase regulating chlorogenic acid in Lonicera japonica Thunb., which were respectively named LjPAL1, LjPAL2, LjPAL3, LjPAL4, and LjPAL5, by scanning proteome database of Lonicera japonica Thunb. based on the amino acid sequences of 33 chlorogenic acid proteins from 17 species, through NCBI-BLAST,phylogenetic analysis and domain analysis. The subcellular localization, physical properties and chemical analysis showed that the five candidate genes encoded proteins with 686~714 amino acids, theoretical isoelectric points were 5.85~6.26, and they were stable hydrophilic protein localized in the cytoplasm. This study provides genetic resources for molecular marker-assisted breeding of Lonicera japonica Thunb., and provides a basis for pathways research of chlorogenic acid in Lonicera japonica Thunb..

关 键 词：金银花(Lonicera japonica Thunb.) 绿原酸 同源基因 生物信息学分析 

分 类 号：S567.79[农业科学—中草药栽培]