日本结缕草miR156和SBP基因的全基因组鉴定及分析  

Genome-wide Identification and Analysis of miR156 and SBP in Zoysia japonica

作　　者：谢琦 高雷 Xie Qi;Gao Lei(Guangdong Provincial Key Laboratory for Plant Epigenetics,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,518060;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen,518060)

机构地区：[1]深圳大学生命与海洋科学学院,广东省植物表观遗传学重点实验室,深圳518060 [2]深圳大学光电工程学院,光电子器件与系统教育部重点实验室,深圳518060

出　　处：《分子植物育种》2024年第22期7413-7420,共8页Molecular Plant Breeding

基　　金：广东省创新创业团队项目(2014ZT05S078);深圳市基础研究面上项目(20200812151848001)共同资助。

摘　　要：SQUAMOSA启动子结合蛋白(SQUAMOSA-promoter binding like proteins, SBPs/SPLs)是植物中一类重要的转录因子家族,它的部分成员在microRNA156 (miR156)的调控下参与到植物各类的生物学过程,包括逆境胁迫、花芽分化以及花形态建成等。本研究以日本结缕草(Zoysia japonica)基因组为基础,通过对SBP蛋白保守结构域的相似性分析鉴定到了20个Zj SBP家族成员,蛋白的氨基酸长度为203~902 aa,相对分子质量介于22~96 k D之间,等电点为5.26~10.24。通过miRNA的预测分析鉴定到了14个日本结缕草Zja-miR156;通过序列特性及靶向分析,发现其中8个SBP成员为miR156的靶基因。对日本结缕草、二穗短柄草(Brachypodium distachyon)、大麦(Hordeum vulgare)、小麦(Triticum aestivum)、水稻(Oryza sativa)和拟南芥(Arabidopsis thaliana) SBP蛋白进行系统进化学分析,发现它们可被划分为8个进化亚群。对日本结缕草miR156、SBP成员的鉴定及分析有助于深入挖掘miR156和SBP生物学功能,并为提高结缕草分子改良育种提供数据基础。SQUAMOSA promoter binding like proteins(SBPs/SPLs) are an important family of transcription factors in plants. Some of its members are regulated by microRNA156(miR156) to participate in various plant biological processes, including stress, flower bud differentiation, and flower morphogenesis. In this study, based on the genome of Zoysia japonica, 20 members of the Zj SBP family were identified by similarity analysis of the conserved domain of SBP protein. The amino acid length of the prot eins ranged from 203 to 902 aa and their relative molecular weight ranged from 22 to 96 kD. The isoelectric point ranges from 5.26 to 10.24. 14 Z. japonica Zja-miR156 were identified by miRNA prediction analysis. Through sequence characterization and targeting analysis, 8 SBP members were found to be the target genes of miR156. The SBP proteins from Z. japonica,Brachypodium distachyon, Hordeum vulgare, Triticum aestivum, Oryza sativa and Arabidopsis thaliana shows that they can be classified into eight subgroups by physiology tree analysis. The identification and analysis of miR156and SBP members of Z. japonica are helpful to further explore the biological functions of miR156 and SBP, and provide data basis for improving molecular breeding of Z. japonica.

关 键 词：SBP家族 日本结缕草 miR156 靶基因 

分 类 号：S688.4[农业科学—观赏园艺]