益母草碱对运动性骨关节损伤大鼠的保护作用及其机制研究  

Protective E ffects and Mechanisms of Leonurine on Exercise-Induced Osteoarthritis in Rats

作　　者：张寅 Zhang Yin(Ministry of Sports,Zhengzhou Vocational College of Finance and Taxation,Zhengzhou,450000)

机构地区：[1]郑州财税金融职业学院体育部,郑州450000

出　　处：《分子植物育种》2024年第22期7572-7579,共8页Molecular Plant Breeding

基　　金：教育部人文社会科学研究青年基金项目(23YJC890024)资助。

摘　　要：益母草碱是从益母草中分离到的生物碱,具有抗氧化、抗炎、神经保护、心肌保护、心血管保护等活性。本研究探讨了益母草碱对运动性骨关节损伤大鼠的保护作用及其机制。通过对大鼠进行运动性骨关节损伤模型的建立,并进行益母草碱干预,检测其对疼痛、氧化应激、炎症反应及膝关节软骨损伤的影响。结果显示,与对照组相比,模型组大鼠的疼痛加剧,血清中氧化应激和炎症指标水平升高,膝关节软骨结构破坏,软骨降解相关基因MMP-3和MMP-13表达增加,合成相关基因SOX9和COL2A1表达减少。益母草碱干预显著减轻了大鼠的疼痛,降低了血清中的氧化应激和炎症指标,改善了膝关节软骨的结构,并通过调节AMPK/NF-κB通路上调了SOX9和COL2A1的表达,下调了MMP-3和MMP-13的表达。综上所述,益母草碱通过多重机制对运动性骨关节损伤大鼠具有显著的保护作用,具有潜在的临床应用价值。Zhang Yin;Ministry of Sports, Zhengzhou Vocational College of Finance and Taxation;Leonurine(LN) is an alkaloid isolated from Leonurus artemisia, exhibiting activities such as antioxidant, anti-inflammatory, neuroprotective, cardioprotective, and cardiovascular protective effects. This study investigates the protective effects and mechanisms of leonurine on exercise-induced osteoarthritis in rats. A rat model of exercise-induced osteoarthritis was established, and the effects of leonurine intervention on pain, oxidative stress,inflammatory response, and knee cartilage damage were evaluated. Results indicated that compared to the control group, the model group showed increased pain, elevated serum levels of oxidative stress and inflammatory markers, structural damage to knee cartilage, increased expression of cartilage degradation-related genes MMP-3 and MMP-13, and decreased expression of cartilage synthesis-related genes SOX9 and COL2A1. Leonurine intervention significantly alleviated pain, reduced serum levels of oxidative stress and inflammatory markers, improved the structure of knee cartilage, upregulated the expression of SOX9 and COL2A1, and downregulated the expression of MMP-3 and MMP-13 through the regulation of the AMPK/NF-κB pathway. In conclusion, leonurine exerts significant protective effects on exercise-induced osteoarthritis in rats through multiple mechanisms, highlighting its potential clinical application value.

关 键 词：益母草碱 运动性骨关节损伤 炎症反应 氧化应激 AMPK/NF-κB通路 

分 类 号：R285.5[医药卫生—中药学]