机构地区:[1]华中农业大学动物科学技术学院、动物医学院,武汉430070
出 处:《动物营养学报》2024年第11期7015-7027,共13页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:国家重点研发计划(2021YFD1300405-12)。
摘 要:本试验基于析因法原理,通过将营养需要分为生长需要和维持需要2部分,建立7~12周龄京粉6号蛋鸡氨基酸需要量动态预测模型。生长需要试验选取96只7周龄京粉6号蛋鸡,随机分为6个重复,每个重复16只鸡,均饲喂基础饲粮;试验期为7~12周龄,用于建立氨基酸生长需要量模型。维持需要试验分别于7~8周龄、9~10周龄和11~12周龄各进行1次,每次选取60只蛋鸡,随机分为限饲组和无氮饲粮组,每组6个重复,每个重复5只鸡;每次试验期为7 d,建立氨基酸维持需要量模型。综合氨基酸生长需要量和维持需要量,建立氨基酸需要量动态预测模型。结果表明:1)7~12周龄蛋鸡生长曲线拟合方程为:BW_(t)=3224.649exp[-3.561exp(-0.012 t)](R^(2)=0.999)。式中:BW_(t)为t日龄体重(g);t为日龄。2)随着日龄的增长,7~12周龄京粉6号蛋鸡胴体和羽毛氨基酸模式无显著差异(P>0.05)。3)7~12周龄蛋鸡氨基酸需要量模型为:TAAR={14.207exp[-3.561exp(-0.012 t)-0.012 t]+53.321exp[-7.122exp(-0.012 t)-0.012 t]}×1000×AAc/NPU+{-1.033exp[-3.561exp(-0.012 t)-0.012 t]+44.434exp[-7.122exp(-0.012 t)-0.012 t]}×1000×AAf/NPU+{19.67×{3224.649exp[-3.561exp(-0.012 t)]}^(0.75)×AAm+C}/SID;TAAR=(8×10^(-7)BW^(2)+0.004 BW+0.706)×1000×AAc/NPU+(-4×10^(-6)BW^(2)+0.009 BW-2.936)×1000×AAf/NPU+(19.67×BW^(0.75)×AAm+C)/SID。式中:TAAR为氨基酸需要量(mg/d);AAc为胴体氨基酸模式(%);AAf为羽毛氨基酸模式(%);NPU为蛋白质净利用率(%);AAm为用于维持的氨基酸模式(%);C为肌酐排泄量(mg/d);SID为氨基酸消化率(%);t为日龄;BW为体重(g);BW^(0.75)为代谢体重(g)。综上所述,本试验利用析因法建立了7~12周龄京粉6号蛋鸡氨基酸需要量动态预测模型。Based on the principle of factorial method,a dynamic prediction model of amino acid requirements for Jing Tint 6 laying hens from 7 to 12 weeks of age was established by dividing nutrient requirement into growth requirement and maintenance requirement.In the growth requirement experiment,a total of 967-week-old Jing Tint 6 laying hens were randomly divided into 6 replicates with 16 chickens in each replicate and fed a basal diet.The experiment lasted from 7 to 12 weeks of age and was used to establish the amino acid growth requirement model.The maintenance requirement experiments were conducted once from 7 to 8 weeks of age,9 to 10 weeks of age and 11 to 12 weeks of age,and each 60 laying hens were randomly divided into restricted feeding group and nitrogen-free diet group with 6 replicates in each group and 5 chickens in each replicate.Each experiment lasted for 7 days,and the amino acid maintenance requirement model was established.Combining the growth requirement and maintenance requirement of amino acids,the dynamic prediction model of amino acid requirements was established.The results showed as follows:1)the fitting equation of growth curve of laying hens from 7 to 12 weeks of age was BW_(t)=3224.649exp[-3.561exp(-0.012 t)](R^(2)=0.999),in which BW_(t) was the body weight at t days of age(g),and t was the days of age.2)With the increase of days of age,there were no significant differences in amino acid patterns in the carcass and feathers of laying hens from 7 to 12 weeks of age(P>0.05).3)The amino acid requirement model of laying hens from 7 to 12 weeks of age was TAAR={14.207exp[-3.561exp(-0.012 t)-0.012 t]+53.321exp[-7.122exp(-0.012 t)-0.012 t]}×1000×AAc/NPU+{-1.033exp[-3.561exp(-0.012 t)-0.012 t]+44.434exp[-7.122exp(-0.012 t)-0.012 t]}×1000×AAf/NPU+{19.67×{3224.649exp[-3.561exp(-0.012 t)]}^(0.75)×AAm+C}/SID or TAAR=(8×10^(-7) BW^(2)+0.004 BW+0.706)×1000×AAc/NPU+(-4×10^(-6) BW^(2)+0.009 BW-2.936)×1000×AAf/NPU+(19.67×BW^(0.75)×AAm+C)/SID,in which TAAR was the amino acid requirement(mg/d);AAc
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