光照周期变化对绒山羊血清激素指标、毛囊形态和皮肤组织基因表达的影响  

Effects of Photo Period Change on Serum Hormone Indexes,Hair Follicle Morphology and Skin Tissue Gene Expression of Cashmere Goats

作　　者：毛晨羽 高丽 王储 殷雪蕾 MAO Chenyu;GAO Li;WANG Chu;YIN Xuelei(Baotou Teachers College,Baotou 014030,China)

机构地区：[1]包头师范学院,包头014030

出　　处：《动物营养学报》2024年第11期7145-7160,共16页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：内蒙古自治区创新训练项目(S202310131024);包头师范学院校级科研项目(BSYKJ2022-ZY03)。

摘　　要：本试验旨在探讨光照周期变化对绒山羊血清激素指标、毛囊形态和皮肤组织基因表达的影响。选取18只阿尔巴斯绒山羊,随机分为3组,每组6头。对照组(CG组)采用自然光照周期,短光照周期组(SDPP组)采用光照8 h/d、黑暗16 h/d的光照周期,渐减光照周期组(SPP组)采用光照时间从16 h/d逐渐缩短到8 h/d的光照周期。试验期为60 d。结果表明:1)试验第26~60天,SDPP组的血清褪黑素(MLT)浓度显著高于CG组(P<0.05);试验第52~60天,SPP组的血清MLT浓度显著高于CG组(P<0.05)。试验第46~60天,SDPP组的血清催乳素(PRL)浓度显著低于CG组(P<0.05);试验第56~60天,SPP组血清PRL浓度显著低于CG组(P<0.05)。试验第54~60天,SDPP和SPP组的血清胰岛素样生长因子-1(IGF-1)浓度显著高于CG组(P<0.05)。试验第56~60天,SDPP和SPP组的血清表皮生长因子(EGF)浓度显著高于CG组(P<0.05)。试验第48~60天,SDPP组的血清三碘甲状腺原氨酸(T 3)浓度显著高于CG组(P<0.05);试验第52~60天,SPP组的血清T 3浓度显著高于CG组(P<0.05)。2)试验第60天,SPP组的次级毛囊深度和初级毛球宽度显著高于CG组(P<0.05),次级毛球宽度显著高于CG和SDPP组(P<0.05)。SDPP和SPP组的毛囊中β-连环蛋白(β-catenin)的mRNA相对表达量显著高于CG组(P<0.05),SPP组的毛囊中血小板衍生生长因子A(PDGFA)的mRNA相对表达量显著高于CG和SDPP组(P<0.05)。3)试验第30天,SDPP组的皮肤组织总超氧化物歧化酶(T-SOD)活性显著高于CG和SPP组(P<0.05);试验第60天,SDPP和SPP组的皮肤组织过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性显著高于CG组(P<0.05),皮肤组织丙二醛(MDA)含量显著低于CG组(P<0.05)。试验第60天,SDPP和SPP组的皮肤组织中SOD1、GPx4和CAT的mRNA相对表达量显著高于CG组(P<0.05)。由此可见,短光照和渐减光照周期可以通过影响绒山羊毛囊发育激素指标的分泌和介导关键基因表达同时影响毛囊活动,使在自然条件下的次级�This experiment was to investigated the effects of photo period change on serum hormone indexes,hair follicle morphology and skin tissue gene expression of cashmere goats.Eighteen Albas cashmere goats were selected and randomly divided into three groups with six goats in each group.The control group(CG group)adopted a natural photo period,the short-day photo period group(SDPP group)adopted a photo period of 8 h/d light and 16 h/d darkness,and the shortening photo period group(SPP group)adopted a photo period which the photo period gradually reduced from 16 h/d to 8 h/d.The experiment lasted for 60 days.The results showed as follows:1)during days 26 to 60 of the experiment,the serum melatonin(MLT)concentration of SDPP group was significantly higher than that of CG group(P<0.05);during days 52 to 60 of the experiment,the serum MLT concentration of SPP group was significantly higher than that of CG group(P<0.05).During days 46 to 60 of the experiment,the serum prolactin(PRL)concentration of SDPP group was significantly lower than that of CG group(P<0.05);during days 56 to 60 of the experiment,the serum PRL concentration of SPP group was significantly lower than that of CG group(P<0.05).During days 54 to 60 of the experiment,the serum insulin-like growth factor-1(IGF-1)concentration of SDPP and SPP groups was significantly higher than that of CG group(P<0.05).During days 56 to 60 of the experiment,the serum epidermal growth factor(EGF)concentration of SDPP and SPP groups was significantly higher than that of CG group(P<0.05).During days 48 to 60 of the experiment,the serum triiodothyronine(T 3)concentration of SDPP group was significantly higher than that of CG group(P<0.05);during days 52 to 60 of the experiment,the serum T 3 concentration of SPP group was significantly higher than that of CG group(P<0.05).2)On day 60 of the experiment,the secondary hair follicle depth and primary hairball width of SPP group were significantly higher than those of CG group(P<0.05),and the secondary hairball width was significantly h

关 键 词：绒山羊 光照周期变化 褪黑素 毛囊 长绒激素 皮肤抗氧化状态 

分 类 号：S826[农业科学—畜牧学]