基于铽有机框架材料荧光法检测动物尿液中莱克多巴胺残留  

Detection of Ractopamine Residues in Animal Urine by Fluorescence Method Based on Terbium Organic Framework

作　　者：沈丽[1] 段苏峦 李润娴 赵晓云 贺平丽 SHEN Li;DUAN Suluan;LI Runxian;ZHAO Xiaoyun;HE Pingli(Logistics School,Beijing Wuzi University,Beijing 101149,China;National Key Laboratory of Livestock and Poultry Nutrition and Feeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;College of Biological Sciences,China Agricultural University,Beijing 100193,China)

机构地区：[1]北京物资学院物流学院,北京101149 [2]中国农业大学动物科学技术学院,畜禽营养与饲养全国重点实验室,北京100193 [3]中国农业大学生物学院,北京100193

出　　处：《动物营养学报》2024年第11期7435-7444,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发项目(2023YFD1301501)。

摘　　要：本研究旨在建立一种β-受体激动剂莱克多巴胺的快速检测方法。以三羧酸化合物作为配体,镧系金属铽作为中心离子合成多核镧系金属有机框架材料。该材料在550 nm处出现铽的强发射峰,能够被莱克多巴胺有效猝灭。结果表明:在0.3~30.0μmol/L范围内,莱克多巴胺浓度的对数与荧光强度之间存在良好的线性关系,检出限可达到6 nmol/L;不同种类β-受体激动剂对多核镧系金属有机框架材料荧光猝灭效率的影响很小,表明多核镧系金属有机框架材料对莱克多巴胺检测具有良好的选择性和抗干扰性;将多核镧系金属有机框架材料用于实际样品的检测,在尿样中莱克多巴胺的加标回收率为87.3%~108.7%。因此,多核镧系金属有机框架材料在莱克多巴胺的检测方面具有良好的应用前景。This experiment was conducted to establish a rapid method for the detection of β-receptor agonist ractopamine.The polynuclear lanthanide metal-organic framework was synthesized by using tricarboxylic acid as ligand and lanthanide terbium as central ion.The material exhibited a strong emission peak of terbium at 550 nm,which could be effectively quenched by ractopamine.The results showed that there was a good linear relationship between the logarithm of ractopamine concentration and fluorescence intensity in the range of 0.3 to 30.0μmol/L,and the detection limit could reach 6 nmol/L;the different kinds ofβ-receptor agonists had little effects on the fluorescence quenching efficiency of polynuclear lanthanide metal-organic framework,which indicated that the polynuclear lanthanide metal-organic framework had good selectivity and anti-interference for ractopamine detection;the adding standard recovery rate of ractopamine in urine samples was 87.3% to 108.7% when the polynuclear lanthanide metal-organic framework was used for the detection of actual samples.Therefore,the polynuclear lanthanide metal-organic framework has a good application prospect in the detection of ractopamine.

关 键 词：镧系金属有机框架 莱克多巴胺 荧光猝灭 荧光检测 

分 类 号：S816[农业科学—饲料科学]