胃疡宁丸防治大鼠胃黏膜损伤的作用机制研究  

Effect Mechanism of Chinese Patent Medicine Weiyangning Pill on Preventing and Treating Gastric Mucosal Injury in Rats

作　　者：戴杏珍 朱泽明 王小兰 梁欣仪 覃仁安 李茹柳[1] 胡玲[1] DAI Xingzhen;ZHU Zeming;WANG Xiaolan;LIANG Xinyi;Qin REN'AN;LI Ruliu;HU Ling(Science and Technology Innovation Center,Piwei Institute,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangzhou Baiyunshan Chenliji Pharmaceutical Factory Company,Guangzhou 510288,China)

机构地区：[1]广州中医药大学科技创新中心、脾胃研究所,广州510405 [2]广州白云山陈李济药厂有限公司,广州510288

出　　处：《医药导报》2024年第12期1904-1912,共9页Herald of Medicine

基　　金：“胃疡宁防治实验性胃肠黏膜损伤的作用机制研究”(中医科技横向合同No.20221045)。

摘　　要：目的探讨胃疡宁丸对无水乙醇造模大鼠胃黏膜损伤的修复作用及其机制,并建立高效液相色谱法(HPLC)测定胃疡宁丸的5种主要成分。方法用HPLC检测胃疡宁丸中的芍药苷、补骨脂、白术内酯III、甘草苷和橙皮苷五种成分;将SD雄性大鼠随机分为正常对照组、模型对照组、胃乃安组、胃疡宁丸大剂量组、胃疡宁丸小剂量组;大鼠禁食不禁水24 h,正常对照组和模型对照组灌胃纯水,胃乃安组灌胃胃乃安(3 g·kg^(-1)),受试药组灌胃胃疡宁(3、1.5 g·kg^(-1));2 h后除正常对照组外其余大鼠均用无水乙醇(5 mL·kg^(-1))灌胃建立胃黏膜损伤模型,1 h后,实验取材,观察并计算胃黏膜损伤大体评分,胃黏膜切片苏木精-伊红(HE)染色并计算病理评分;免疫组化法检测胃黏膜相关蛋白表达。结果胃疡宁丸HPLC色谱图可见与5种标准品(芍药苷、补骨脂、白术内酯III、甘草苷和橙皮苷)保留时间相同的吸收峰;胃疡宁组(3、1.5 g·kg^(-1))大鼠胃黏膜损伤大体评分和病理评分均低于模型对照组(P<0.05或P<0.01);胃疡宁(3、1.5 g·kg^(-1))对无水乙醇造模大鼠胃黏膜紧密连接蛋白Claudin-7、黏附连接蛋白E-cadherin和β-catenin、黏蛋白MUC1和MUC5AC、转录因子SOX2蛋白表达降低有改善作用(与模型对照组比较,P<0.05或P<0.01)。结论胃疡宁丸防治无水乙醇所致大鼠胃黏膜损伤的修复作用与其提高紧密连接蛋白、黏附连接蛋白、黏蛋白和转录因子表达有关。Objective To investigate the repairing effect and mechanism of Chinese patent medicine Weiyangning pill on gastric mucosal injury in rats induced by anhydrous ethanol,and to establish a high-performance liquid chromatography(HPLC)method to determine the five main components of Weiyangning pill.Methods The five components of paeoniflorin,psoralen,atractylenolide III,liquiritin and hesperidin in Weiyangning pill were detected by HPLC.SD male rats were randomly divided into normal control group,model control group,Weinaian group,and large and small dose group of Weiyangning pill.All rats were fasted for 24 hours without water fasting.The normal control group and the model control group were given purified water by gavage.While Weinaian group was given Weinaian(3 g·kg^(-1)),the test group were given intragastric perfusion of Weiyangning(3,1.5 g·kg^(-1))respectively.After 2 hours,all the rats,except the normal control group,were intragastrically administered with anhydrous ethanol(5 mL·kg^(-1))to establish the model of gastric mucosal injury.An hour later,the experimental materials were collected,and the gross score of gastric mucosal injury was observed and calculated.The gastric mucosal slices were stained by hematoxylin-eosin(HE)to calculate the pathological scores.Immunohistochemistry was employed to detect the expression of gastric mucosa-related proteins.Results The high-performance liquid chromatogram of Weiyangning pill was obtained,and the absorption peaks with the same retention time as the five standard substances(paeoniflorin,psoralen,atractylenolide Ⅲ,liquiritin and hesperidin)were observed.The general score and pathological score of gastric mucosal injury in Weiyangning groups(3,1.5 g·kg^(-1))were lower than those of the model control group(P<0.05 or P<0.01).Weiyangning pill(3,1.5 g·kg^(-1))ameliorated the decrease expression of tight junction protein(Claudin-7),adhesion junction proteins(E-cadherin andβ-catenin),mucins(MUC1 and MUC5AC),and the gastric transcription factor SOX2 in the gastric muc

关 键 词：胃疡宁丸 胃黏膜损伤 胃黏膜屏障 细胞连接 转录因子 

分 类 号：R975[医药卫生—药品] R573[医药卫生—药学]