锁阳乙酸乙酯提取物对AD模型秀丽隐杆线虫脂质代谢的影响  

Effects of Ethyl Acetate Extract of Suoyang(Cynomorium Songaricum)on Lipid Metabolism of AD model of Caenorhabditis Elegans

作　　者：张煦雅 符琰 李雪 张雅丽 李玲玲 鲁艺 ZHANG Xuya;FU Yan;LI Xue;ZHANG Yali;LI Lingling;LU Yi(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing China 100029)

机构地区：[1]北京中医药大学中医学院,北京100029

出　　处：《中医学报》2024年第12期2640-2647,共8页Acta Chinese Medicine

基　　金：国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023256)。

摘　　要：目的:探讨锁阳乙酸乙酯提取物(the ethyl acetate extract of cynomorium songaricum,ECS)对阿尔茨海默病(Alzheimer′s disease,AD)模型秀丽隐杆线虫的影响,包括抗衰老、抗氧化应激及调节脂质代谢。方法:采用限时产卵法使线虫同步化,将L4期野生型线虫作为空白组(N2组),突变体线虫随机分为模型组(CL4176组)和ECS组(CL4176+ECS组)。ECS的浓度为0.4 g·L^(-1),给药3 d。通过寿命实验、慢性百草枯应激实验、急性热应激实验、头摆实验、正弦运动实验、体宽体长实验检测线虫的生存情况。采用荧光分子探针(H2DCF-DA)检测线虫的活性氧(reative oxygen species,ROS)水平,油红O实验检测脂肪含量,qRT-PCR法检测nhr-49、acs-2、sodh-1、fat-7表达水平。结果:寿命实验、慢性百草枯应激实验、急性热应激实验均显示,CL4176组线虫的平均寿命较N2组缩短,经ECS治疗后延长(P<0.01)。与N2组比较,CL4176组线虫的头摆频率、正弦运动频率降低(P<0.01)。与CL4176组比较,CL4176+ECS组线虫的头摆频率、正弦运动频率提高(P<0.01)。与N2组比较,CL4176组线虫的平均/相对体宽、平均/相对体长增加(P<0.001)。与CL4176组比较,CL4176+ECS组线虫的平均/相对体宽、平均/相对体长减少(P<0.001)。CL4176组线虫的ROS水平、脂肪含量、游离脂肪酸(non-esterified fatty acids,NEFA)含量较N2组升高,经ECS治疗后降低(P<0.05)。qRT-PCR显示,与N2组比较,CL4176组线虫的fat-7 mRNA、acs-2 mRNA、sodh-1 mRNA水平升高,nhr-49 mRNA水平降低(P<0.01)。与CL4176组比较,CL4176+ECS组线虫的acs-2 mRNA、sodh-1 mRNA水平降低,nhr-49 mRNA水平升高(P<0.01)。结论:ECS对AD模型秀丽隐杆线虫具有抗衰老、提高认知功能的作用,其机制可能与抗氧化、调节脂质代谢有关。Objective:To investigate the effects of the ethyl acetate extract of Suoyang(cynomorium songaricum,ECS)on the Alzheimer′s disease(AD)model of caenorhabditis elegans,including anti-aging,anti-oxidative stress and regulation of lipid metabolism.oxidative stress and regulation of lipid metabolism.Methods:The nematodes were synchronized by a time-limited spawning method,and the L4 wild-type nematodes were used as a blank group(N2 group),while the mutant nematodes were randomly divided into a model group(CL4176 group)and an ECS group(CL4176+ECS group).the ECS was administered at a concentration of 0.4 g·L^(-1)for 3 d.The nematodes were tested by the longevity assay,the chronic paraquat stress assay,the acute heat stress assay,the head-pendulum assay,the sine wave assay,the body width and body length assay,and the body length and body width assay.movement experiment,and body width and length experiment to detect the survival of nematodes.Fluorescent molecular probes(H2DCF-DA)were used to detect the level of reactive oxygen species(ROS)in the nematodes,oil red O assay was used to detect the fat content,and qRT-PCR was used to detect the expression levels of nhr-49,acs-2,sodh-1,and fat-7.Results:Lifespan experiment,chronic paraquat stress experiment,and acute heat stress experiment showed that the average lifespan of nematodes in CL4176 group was shorter than that in N2 group,which was prolonged after ECS treatment(P<0.01).Compared with the N2 group,the head bobbing frequency and sinusoidal movement frequency of nematodes in the CL4176 group were reduced(P<0.01).Compared with the CL4176 group,the head bobbing frequency and sinusoidal movement frequency of nematodes in the CL4176+ECS group increased(P<0.01).Compared with the N2 group,the mean body width and mean body length of nematodes in the CL4176 group increased(P<0.001).The mean body width and mean body length of nematodes in the CL4176+ECS group decreased compared with the CL4176 group(P<0.001).The ROS level,fat content,and NEFA content of nematodes in the CL4176

关 键 词：锁阳 阿尔茨海默病 锁阳乙酸乙酯提取物 脂质代谢 衰老 氧化应激 锁阳 秀丽隐杆线虫 

分 类 号：R285.5[医药卫生—中药学]