基于化学胁迫和原位培养高效分离放线菌素D产生菌  

Efficient Isolation of Actinomycin D-Producing Bacteria Under Chemical Stress and In-situ Culture Method

作　　者：杨帅 冯鑫 戴璟 李一阳 杨波 YANG Shuai;FENG Xin;DAI Jing;LI Yiyang;YANG Bo(Xinjiang Second Medical College,Karamay 834000,China;College of Sciences and Technology,Korla 841000,China)

机构地区：[1]新疆第二医学院,新疆克拉玛依834000 [2]新疆科技学院,新疆库尔勒841000

出　　处：《生物化工》2024年第5期48-55,共8页Biological Chemical Engineering

基　　金：克拉玛依创新环境建设计划(创新人才)(2022hjcxrc0059,2022hjcxrc0053);新疆第二医学院青年基金项目(QK202206)。

摘　　要：为获取能够代谢放线菌素D的放线菌,以克拉玛依戈壁土壤为材料,借鉴原位培养思路及化学胁迫法对培养基进行改良处理,以对含有特定代谢产物的放线菌实现靶向分离。使用宏基因组技术对样本进行分析,采用高氏一号为基础培养基,目标化合物为放线菌素D,向培养基中额外添加放线菌素D进行化学胁迫,并将配置培养基的纯净水换成土壤浸提液来补充培养基中无法提供的有机物及无机盐。结果成功分离获得能够高效代谢放线菌素D的放线菌2株,编号分别是KRY-1004和KRY-1005,通过16S rDNA鉴定分别为链霉菌属Streptomyces sp.和易变链霉菌属Streptomyces mutabilis。推测通过额外添加与目标产物相同或类似的化学物质,结合化学胁迫和原位培养的思路可以提高具备特定代谢产物的放线菌的分离成功率。In order to obtain actinomycetes capable of metabolizing actinomycin D,the Karamay Gobi soil is used as the material,and the culture medium is improved using in-situ culture ideas and chemical stress methods to achieve the desired actinomycetes with specific metabolites.Using metagenomic technology to analyze samples,the Gao Shi No.1 culture medium is used as basic culture medium,and actinomycin D is added to the culture medium for chemical stress,furthermore,the pure water in the culture medium is replaced with soil extract to supplement the organic matter and inorganic salts that are not provided in the base culture medium.As a result,two strains of actinomycetes capable of metabolizing actinomycin D,namely KRY-1004 and KRY-1005,are successfully isolated,which are identified as Streptomyces sp.and Streptomyces mutabilis,respectively through 16S rDNA analysis.It is speculated that the success rate of isolating actinomycetes with specific metabolites can be improved by adding additional chemicals that are same or similar to the target product,along with chemical stress and in situ culture.

关 键 词：放线菌 放线菌素D 分离 化学胁迫 制备 

分 类 号：Q939.92[生物学—微生物学]