冬虫夏草菌对砷耐受和富集特性的研究  

Study on Tolerance and Enrichment of Arsenic in Ophiocordyceps sinensis

作　　者：何丽 周博 肖凡 窦晨曦 杨欢 谢放[1] HE Li;ZHOU Bo;XIAO Fan;DOU Chenxi;YANG Huan;XIE Fang(School of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070)

机构地区：[1]兰州交通大学,生物与制药工程学院,兰州730070

出　　处：《中国农学通报》2024年第33期111-117,共7页Chinese Agricultural Science Bulletin

基　　金：中国国家自然科学基金会“通过转录组分析研究冬虫夏草菌子实体发育启动的关键基因”(31560003)。

摘　　要：本研究旨在探讨冬虫夏草菌对砷(As)的耐受性及富集特性,为评估和解决冬虫夏草含As量的安全性问题提供参考依据。以冬虫夏草菌为试验材料,将不同浓度的As添加到固液培养基中培养一段时间后,检测冬虫夏草菌生长特性及As富集特性相关指标,同时应用Logistic回归法构建As富集回归模型。结果表明:低浓度(1~2 mg/L)As对冬虫夏草菌落、菌丝形态、菌丝生长速度及干重几乎没有影响,而高浓度(高于3 mg/L)As会严重影响菌落形态及菌丝的生长。随着不同As浓度处理时间的延长,液体培养基中As含量呈持续下降的趋势,而菌丝体中As含量呈持续增加的趋势,富集系数呈现出持续降低的趋势,富集系数浮动区间为43.92%~128.22%,这说明冬虫夏草菌以主动吸收的方式富集低浓度的As。不同浓度As处理冬虫夏草菌40 d后,菌丝对As的富集均达到稳定状态。Logistic回归模型结果显示,冬虫夏草菌对As的富集饱和点为1.68 mg/kg DW。冬虫夏草能够主动吸收低浓度的As,其富集饱和点为1.68 mg/kg DW。By studying the tolerance and enrichment characteristics of Ophiocordyceps sinensis(O.sinensis)to arsenic(As),reference for the evaluation and solution of As content safety in O.sinensis was provided.Taking O.sinensis as the experimental material,different concentrations of As were added into the solid and liquid medium to detect the growth characteristics of O.sinensis and the related indicators of As enrichment characteristics,and the As enrichment regression model was constructed by Logistic regression method.The results showed that low concentration(1-2 mg/L)As had little effect on colony,mycelial morphology,mycelial growth rate and dry weight of O.sinensis,while high concentration(>3 mg/L)As had serious effect on colony morphology and mycelial growth.With the extension of treatment time at different As concentrations,the As content in liquid culture medium showed a trend of continuous decline,while the As content in mycelium showed a trend of continuous increase,and the enrichment coefficient showed a continuing downward trend,with the enrichment coefficient floating range of 43.92-128.22%,suggesting O.sinensis enriched As by active absorption way.After the treatment of O.sinensis with different concentrations of As for 40 d,the concentration of As in mycelium reached a stable state.Logistic regression model showed that the enrichment saturation point of O.sinensis for As was 1.68 mg/kg DW.O.sinensis could actively absorb low concentration As,and its enrichment saturation point was1.68 mg/kg DW.

关 键 词：冬虫夏草菌 生长特性 砷 耐受性 富集特性 LOGISTIC回归模型 

分 类 号：S646.9[农业科学—蔬菜学]