Efficient Breeding of Early-Maturing Rice Cultivar by Editing Hd6 via CRISPR/Cas9  

作　　者：CHEN Zhihui TAO Yajun XU Yang WU Jingjing WANG Fangquan LI Wenqi JIANG Yanjie FAN Fangjun LI Xia ZHU Jianping ZHU Qian-Hao YANG Jie 

机构地区：[1]Key Laboratory of Germplasm Innovation in Downstream of Huaihe River,Ministry of Agriculture and Rural Affairs/Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [2]Zhongshan Biological Breeding Laboratory,Nanjing 210014,China [3]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China [4]Institute of Life Science,Jiangsu University,Zhenjiang 212013,China [5]Commonwealth Scientific and Industrial Research Organisation,Agriculture and Food,GPO Box 1700,Canberra ACT 2601,Australia

出　　处：《Rice science》2024年第6期629-633,I0001-I0005,共10页水稻科学（英文版）

基　　金：supported by the Ministry of Agriculture and Rural Affairs of China,the Jiangsu Provincial Key Research and Development Program (Grant No.BE2022383);the Natural Science Foundation of Jiangsu Province,China (Grant No.BK20212010);the STI 2030-Major Projects (Grant No.2023ZD04066);Jiangsu Agricultural Science and Technology Innovation Fund,China (Grant No.CX (22)3138);Zhongshan Biological Breeding Laboratory,China (Grant No.ZSBBL-KY2023-04-1)。

摘　　要：Rice heading date,or flowering time,is important for yield optimization and regional adaptation.While many genes controlling this trait in rice are known,breeders often need only slight adjustments.Our study used the CRISPR/Cas9 method to edit the Hd6 gene in the early-maturing,late-season japonica rice cultivar Nanjing 46(NJ46),creating three mutants with reduced flowering times of 9-12 d under natural conditions in Nanjing city,Jiangsu Province,China.These mutants showed higher Hd3a and RFT1 expression levels without compromising yield or eating and cooking quality,demonstrating that Hd6 gene editing is an effective precision breeding tool for shortening heading date without laborious traditional methods.

关 键 词：CRISPR/Cas9 HEADING BREEDING 

分 类 号：S511[农业科学—作物学]