Histone Acetyltransferase GCN5 Regulates Rice Growth and Development and Enhances Salt Tolerance  

作　　者：XUE Chao ZHAO Xinru CHEN Xu CAI Xingjing HU Yingying LI Xiya ZHOU Yong GONG Zhiyun 

机构地区：[1]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Agricultural College,Yangzhou University,Yangzhou 225009,China [2]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,China [3]School of Agronomy and Horticulture,Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang 212499,China

出　　处：《Rice science》2024年第6期688-699,I0015,共13页水稻科学（英文版）

基　　金：supported by the Key R&D Program of Jiangsu Province (Grant No.BE2022335);the Jiangsu Province Government (Grant No.JBGS[2021]001);the Natural Science Foundation of Jiangsu Province (Grant No.BK20230295);the Project of Zhongshan Biological Breeding Laboratory (Grant No.BM2022008-02);the Outstanding Youth Fund of Jiangsu Province (Grant No.BK20230013);the Priority Academic Program Development of Jiangsu Higher Education Institutions in China。

摘　　要：Histone acetylation is indispensable in the process of crops resisting abiotic stress,which is jointly catalyzed by histone acetyltransferases and deacetylases.However,the mechanism of regulating salt tolerance through histone acetyltransferase GCN5 is still unclear.We revealed that GCN5 can catalyze the acetylation of canonical H3 and H4 lysine residues both in vivo and in vitro in rice.The knockout mutants and RNA interference lines of Os GCN5 exhibited severe growth inhibition and defects in salt tolerance,while the over-expression of Os GCN5 enhanced the salt tolerance of rice seedlings,indicating that Os GCN5 positively regulated the response of rice to salt stress.RNA-seq analysis suggested Os GCN5 may positively regulate the salt tolerance of rice by inhibiting the expression of Os HKT2;1 or other salt-responsive genes.Taken together,our study indicated that GCN5 plays a key role in enhancing salt tolerance in rice.

关 键 词：GCN5 histone acetyltransferase salt tolerance Oryza sativa 

分 类 号：S511[农业科学—作物学]