大豆GmMATH基因家族鉴定与盐胁迫下的表达模式分析  

Identification of GmMATH family genes and analysis of expression pattern under salt stress in soybean(Glycine max)

作　　者：何敬宇 王裕东 张薇 程志远 HE Jingyu;WANG Yudong;ZHANG Wei;CHENG Zhiyuan(State Key Laboratory of Black Soils Conservation and Utilization/Key Laboratory of Soybean Molecular Design and Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China;College of Advanced Agricultural Science,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院东北地理与农业生态研究所,大豆分子设计育种重点实验室/黑土地保护与利用全国重点实验室,吉林长春130102 [2]中国科学院大学现代农业科学院,北京100049

出　　处：《土壤与作物》2024年第4期448-461,共14页Soils and Crops

基　　金：吉林省科技发展计划项目(20230101166JC);国家自然科学基金项目(32272102).

摘　　要：植物MATH基因参与发育、激素信号转导和非生物胁迫等过程,但是大豆GmMATH基因未见详细的研究报道。本文在大豆中鉴定到40个保守的GmMATH基因,根据蛋白相似性分为6个亚家族。对全部GmMATH基因上游2kb启动子上的顺式调控元件进行分析,发现431个与植物发育、应激反应相关的顺式调控元件,表明GmMATH基因参与调节发育和响应胁迫等过程。转录组数据分析显示,GmMATH基因参与大豆不同阶段生长发育过程,并发现32个GmMATH基因在盐胁迫下表达水平会发生显著变化;其中GmMATH01和GmMATH13基因在盐耐受材料中表达明显高于盐敏感材料,表明大豆GmMATH基因表达模式与大豆盐碱耐性相关。本研究为深入解析大豆GmMATH基因在响应外界胁迫和发育调控中的作用提供了重要依据。In plants,the MATH gene is implicated in development,hormone signal transduction,and abiotic stress responses.However,the detailed information regarding the GmMATH gene in soybean has not been reported extensively.In this study,we identified 40 conserved GmMATH genes in soybean.These genes can be categorized into 6 subfamilies based on their protein similarity.Furthermore,an analysis of the 2kb promoter region upstream of all GmMATH genes revealed the presence of 431 cisregulatory elements is associated with plant development and stress response pathways.These findings provide evidence for the involvement of GmMATH genes in developmental regulation and defense responses.Transcriptome data analysis demonstrated that GmMATH genes are engaged at various developmental stages in soybean.Notably,under salt stress conditions,significant changes of expression levels about 32 GmMATH genes are observed;specifically,higher expression levels of GmMATH01 and GmMATH13 are detected in salt-tolerant materials compared to salt-sensitive materials.These findings suggest a correlation between the expression pattern of GmMATH genes and saline-alkali tolerance in soybean.Overall,our study provides a crucial foundation for further investigations into the role played by the GmMATH gene during external stress responses and developmental regulation processes.

关 键 词：大豆 MATH家族 生物信息学分析 表达模式 

分 类 号：S565.1[农业科学—作物学]