介孔有机硅中空纳米球固定尿酸酶用于尿酸检测  

Mesoporous Organosilicon Hollow Nanospheres Immobilized Uricase for the Determination of Uric Acid

作　　者：纪赫鸣 张宇航 苗婷婷[1] 王怡倩 于笑蕊 王春艳[1] 王润伟[2] JI Heming;ZHANG Yuhang;MIAO Tingting;WANG Yiqian;YU Xiaorui;WANG Chunyan;WANG Runwei(School of Public Health,Jilin University,Changchun 130021,China;State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学公共卫生学院,长春130021 [2]吉林大学无机合成与制备化学国家重点实验室,长春130012

出　　处：《高等学校化学学报》2024年第12期1-9,共9页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20210204008YY,20240101180JC)资助.

摘　　要：采用一步生长诱导腐蚀法合成了介孔有机硅中空纳米球(MONs),并用其固定尿酸酶(Uricase),形成了介孔有机硅中空纳米球固定化的尿酸酶(MONs-uricase).采用扫描电子显微镜、透射电子显微镜、红外光谱、氮气吸附-脱附测试和热重分析对MONs和MONs-uricase进行了表征.结果显示,尿酸酶被固定于MONs的介孔结构中,固定率超过90%.MONs具有介孔通道和空心结构,吸附性能良好.MONs-uricase的热稳定性和pH稳定性均比游离尿酸酶高.在血清尿酸(UA)检测中,在0.01~1.00mg/mL范围内MONs-uricase的吸光度与浓度具有良好的线性关系,且特异性良好.MONs-uricase可通过离心回收,在重复使用20次后仍具有约50%的活性,具有良好的可重复利用性.MONs在生物酶的固定化和循环利用方面有较好的应用前景.Mesoporous organosilicon hollow nanospheres(MONs)were synthesized through a one-step growthinduced etching method,and then uricase was successfully immobilized onto MONs,forming MONs-uircase.Comprehensive characterizations of MONs and MONs-uricase were performed using scanning electron microscopy(SEM),transmission electron microscopy(TEM),infrared spectroscopy,N2 adsorption-desorption measurements,and thermogravimetric analysis(TGA).The results indicated that uricase was successfully immobilized within the mesoporous structure of MONs,with an immobilization efficiency exceeding 90%.MONs possessed excellent adsorp⁃tion properties due to their mesoporous channels and hollow structures.Compared to free uricase,MONs-uricase exhibited enhanced thermal and pH stability.In the detection of serum uric acid(UA),MONs-uricase demonstrated a good linear relationship within the UA concentration range of 0.01—1.00 mg/mL,along with high specificity.MONs-uricase could be readily recovered through centrifugation,and the recovered MONs-uricase maintained high enzymatic activity after multiple cycles of use,retaining 50% enzymatic activity even after 20 cycles,indicating its excellent reusability.MONs offered promising application prospects in the immobilization and recycling of biological enzymes.

关 键 词：介孔有机硅中空纳米球 尿酸酶固定 尿酸检测 

分 类 号：O613[理学—无机化学] R115[理学—化学]