基于石墨烯/金纳米粒子/碳化钛复合材料构建双室酶生物燃料电池自供能葡萄糖生物传感器  

A Double-Chamber Enzymatic Biofuel Cells-based Self-powered Glucose Biosensor Based on Graphene/Gold Nanoparticles/Titanium Carbide Nanocomposite

作　　者：李世宣 蒙化 尹学虎 易锦飞 马丽红 张艳丽[1] 王红斌[1] 杨文荣 庞鹏飞[1] LI Shixuan;MENG Hua;YIN Xuehu;YI Jinfei;MA Lihong;ZHANG Yanli;WANG Hongbin;YANG Wenrong;PANG Pengfei(Functional Nanomaterial-based Chemical and Biological Sensing Technology Innovation Team of Department of Education of Yunnan Province,Yunnan Minzu University,Kunming 650504,China;School of Life and Environmental Sciences,Deakin University,Geelong 3217,Australia)

机构地区：[1]云南民族大学,云南省教育厅功能纳米材料基化学生物传感科技创新团队,昆明650504 [2]澳大利亚迪肯大学生命与环境科学学院,吉朗3217

出　　处：《高等学校化学学报》2024年第12期31-39,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21565031,21665027);云南省科技厅应用基础研究基金(批准号:202001AT070012);云南民族大学研究生科研创新基金(批准号:2024SKY135)资助.

摘　　要：基于酶生物燃料电池(EBFCs)构建的自供能电化学传感装置具有结构简单、易于小型化及无需外部电源等优势,在生物传感、环境监测和临床诊断等领域具有潜在的应用前景.本文利用石墨烯/金纳米粒子/碳化钛(rGO/AuNPs/Ti_(3)C_(2))纳米复合材料修饰玻碳电极(rGO/AuNPs/Ti_(3)C_(2)/GCE)作为EBFCs阴极,在其表面进一步固定葡萄糖氧化酶(GOx),制得EBFCs生物阳极,进而通过组装EBFCs生物阳极和阴极构建了双室酶生物燃料电池自供能葡萄糖生物传感器(EBFCs-SPGB).当阳极室存在目标物葡萄糖时,生物阳极表面固定的GOx发生酶促反应,催化葡萄糖产生电子并经外电路到达阴极,导致阴极表面Fe(CN)_(6)^(3-)发生还原反应,从而产生电化学响应信号.rGO/AuNPs/Ti_(3)C_(2)纳米复合材料具有优异的导电性、生物相容性和大的比表面积,其协同效应可显著提高GOx负载量和有效促进电子在电极表面的传递.构建的EBFCs-SPGB最大功率输出信号与葡萄糖浓度在0.3~10 mmol/L范围内呈良好的线性关系,检出限为0.1 mmol/L(S/N=3),可用于人体血清样本中葡萄糖浓度的检测分析.Enzymatic biofuel cells(EBFCs)-based self-powered sensing device has the advantages of simple structure,easy miniaturization,and no need for external power supply.It exhibits potential application prospects in clinical diagnosis,environmental monitoring,biosensing,and other fields.Graphene/gold nanoparticles/titanium carbide(rGO/AuNPs/Ti_(3)C_(2))nanocomposite modified glassy carbon electrode(GCE)was used as cathode of EBFCs(rGO/AuNPs/Ti_(3)C_(2)/GCE).The bioanode of EBFCs was prepared via immobilization of glucose oxidase(GOx)on the surface of rGO/AuNPs/Ti_(3)C_(2)/GCE.A double-chamber enzymatic biofuel cells-based self-powered glucose biosensor(EBFCs-SPGB)was constructed by combining as-prepared bioanode and cathode in supporting electrolyte separated with a Nafion membrane.In the presence of target glucose,the GOx fixed on the surface of the bioanode promotes an enzymatic reaction.The electrons generated by catalyzing glucose transferred to the cathode through an external circuit,resulting in a reduction reaction on the cathode surface and generating an electrochemical response signal.Due to the excellent conductivity,biocompatibility,and large specific surface area of rGO/AuNPs/Ti_(3)C_(2) nanocomposite,the synergistic effect of nanocomposite can significantly increase loading amount of GOx and effectively promote electron transfer on the electrode surface.The maximum power output signal of the constructed EBFCs-SPGB shows a good linear relationship with glucose concentration in the range of 0.3—10 mmol/L,with a detection limit of 0.1 mmol/L(S/N=3),which can be applied to analysis of glucose concentration in human serum samples.

关 键 词：葡萄糖 纳米复合材料 酶生物燃料电池 自供能电化学传感器 

分 类 号：O657.1[理学—分析化学]