新型稀土近红外荧光探针用于活体多重成像  

Novel Rare Earth Near-infrared Fluorescent Probes for in vivo Multiplexed Imaging

作　　者：李红雨 张洪新 LI Hongyu;ZHANG Hongxin(Laboratory of Advanced Materials,Fudan University,Shanghai 200438,China)

机构地区：[1]复旦大学先进材料实验室,上海200438

出　　处：《高等学校化学学报》2024年第12期47-56,共10页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22104017)资助.

摘　　要：提出了激发/发射双维度的荧光编码和活体多通道成像技术,开发了用于三通道活体成像的稀土近红外二区(NIR-Ⅱ,1000~1700 nm)荧光探针α-NaYbF_(4)∶8%Tm@NaYbF_(4)@NaYF_(4)(Yb/Tm),α-NaYbF_(4)∶2%Er@NaYbF_(4)@NaYF_(4)(Yb/Er)和β-NaErF_(4)@NaYF_(4)(Er@Y).其中,纳米颗粒Yb/Tm在915 nm激光激发下,可发射出1640 nm的长波近红外二区(NIR-Ⅱ-L,1500~1900 nm)荧光,在808 nm激光激发下几乎无荧光输出;Yb/Er在915 nm激光激发下,可发射出1532 nm的NIR-Ⅱ-L荧光,在808 nm激光激发下几乎无荧光输出;Er@Y在808 nm激光激发下,可发射出1525 nm的NIR-Ⅱ-L荧光,在915 nm激光激发下仅有微弱信号输出.通过稀土核壳纳米结构的设计实现了荧光强度216倍的增强,在不同功率激发下,纳米颗粒可保持独立激发和独立发射.基于上述纳米颗粒的独特光学性质,实现了三通道信息光学编码和活体高分辨检测,该方法为光学信息存储和活体多重分析提供了新的工具和思路.Fluorescence encoding and in vivo multiplexed imaging with dual-dimensions of excitation and emission are proposed.A series of second near-infrared(NIR-Ⅱ,1000—1700 nm)fluorescent probes[α-NaYbF_(4)∶8%Tm@NaYbF_(4)@NaYF_(4)(Yb/Tm),α-NaYbF_(4)∶2%Er@NaYbF_(4)@NaYF_(4)(Yb/Er)andβ-NaErF_(4)@NaYF_(4)(Er@Y)]were developed for triple-channel in vivo imaging.Among them,Yb/Tm nanoparticles can emit 1640 nm long-wave second near-infrared fluorescence(NIR-Ⅱ-L,1500—1900 nm)under 915 nm laser excitation while no fluorescence signal can be detected under 808 nm excitation.Yb/Er can emit NIR-Ⅱ-L fluorescence at 1532 nm under excitation of 915 nm laser while no fluorescence signal can be detected under 808 nm excitation.In contrast,Er@Y nanoparticles excited by an 808 nm laser can emit NIR-Ⅱ-L fluorescence at 1525 nm,while a very weak signal can be detected under the excitation of 915 nm.The design of rare earth core-shell nanostructures successfully enhances the fluores⁃cence intensity by 216 times.Under different power density excitations,the nanoparticles still maintain excellent optical properties of independent excitation/emission.Based on the unique optical properties of these nanoparticles,optical encoding and in vivo high-resolution detection with three channels have been achieved,providing new tools and solutions for optical information storage and in vivo multiplexed analysis.

关 键 词：稀土荧光探针 近红外二区 活体成像 多重成像 

分 类 号：O657[理学—分析化学]