间伐恢复对松栎混交林表层土壤酶活性及酶化学计量的影响  

Effect of thinning restoration on enzyme activity and enzyme stoichiometry in the topsoil of oak-pine mixed forest

作　　者：谭蕊 于水强[1,2] 李玉 王祥福 徐新颖 李愿会 王维枫 TAN Rui;YU Shuiqiang;LI Yu;WANG Xiangfu;XU Xinying;LI Yuanhui;WANG Weifeng(Regional Ecological Sustainable Development Research Center,College of Ecology and Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Co-Innovation Center of the Sustainable Forestry in Southern China,College of Ecology and Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Key Laboratory National Forestry Administration on Ecological Hydrology and Disaster Prevention in Arid Regions,Northwest Surveying and Planning Institute of National Forestry and Grassland Administration,Xi’an 710048,Shaanxi,China)

机构地区：[1]南京林业大学生态与环境学院区域生态可持续发展研究中心,江苏南京210037 [2]南京林业大学生态与环境学院南方现代林业协同创新中心,江苏南京210037 [3]国家林业和草原局西北调查规划院旱区生态水文与灾害防治国家林业局重点实验室,陕西西安710048

出　　处：《浙江农林大学学报》2024年第6期1201-1210,共10页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(32071763);国家林业和草原局西北调查规划院2021年科技创新资助项目(XBJ-KJCX-2021-15)。

摘　　要：【目的】探讨间伐后不同恢复时间下秦岭松栎混交林土壤理化性质和土壤胞外酶活性的变化,研究该地区抚育间伐措施对森林生态系统养分循环过程,为制定森林可持续经营方案及合理的生态恢复措施提供理论依据。【方法】采用空间代替时间的方法,对松栎混交林间伐后不同恢复时间(5、13 a)的表层土壤(0~10 cm)理化性质和胞外酶活性进行测定,并计算各处理样地酶化学计量比和酶矢量,以未间伐为对照。【结果】①间伐后土壤pH提高,恢复13 a时土壤全磷、微生物量碳和微生物量氮质量分数显著增加(P<0.05),土壤有效氮质量分数降低。土壤有机碳质量分数在恢复5 a时显著下降(P<0.05),在13 a时逐渐恢复至间伐前水平。②间伐恢复显著降低了β-木糖苷酶、氮获取酶和酸性磷酸酶的活性(P<0.05),提高了β-葡糖苷酶活性;酚氧化物酶和过氧化物酶活性在间伐恢复初期(5 a)表现为降低趋势,13 a时恢复到间伐前水平。③间伐恢复13 a时土壤碳获取酶/氮获取酶比值(E_(C/N))、土壤碳获取酶/磷获取酶比值(E_(C/P))和酶矢量长度显著提高(P<0.05),间伐恢复5 a时E_(C/P)和土壤氮获取酶/磷获取酶比值(E_(N/P))显著提高,同时酶矢量角度也显著降低(P<0.05)。【结论】随间伐恢复时间延长,土壤养分、有机碳和氧化酶活性呈现逐渐恢复的趋势;pH是影响土壤酶活性及酶矢量变化的关键因子。间伐导致土壤微生物在初期恢复阶段的磷限制有所缓解,后期并未改变受碳、磷共同限制的状况。[Objective]This study,with an investigation of the effects of different recovery periods after thinning on soil physical-chemical proprieties and extracellular enzyme activities in oak-pine mixed forests in the Qinling Mountains,is aimed to better understand the nutrient cycling processes under thinning treatments,providing basis for developing programs of sustainable forest management and exploring better ecological restoration measures.[Method]First,pace-for-time substitution was employed to explore the effects of thinning restoration process(5 and 13 years,no thinning as the control)on soil physical-chemical proprieties and enzyme activity changes in the surface layer at 0−10 cm depth.Then the enzyme stoichiometric ratios and enzyme vectors were calculated for each treatment.[Result](1)The total phosphorus,microbial biomass carbon,and microbial biomass nitrogen contents in the soil increased significantly,whereas the inorganic nitrogen content decreased in the 13-year restoration(P<0.05)and the soil pH increased in the 5-year and 13-year restorations;following a 13-year restoration,the soil organic carbon content progressively recovered to pre-thinning levels after declining dramatically in the 5-year restoration(P<0.05).(2)Thinning significantly increased the activity ofβ-glucosidase(BG),while decreasing the activities ofβ-xylosidase(BX),nitrogen acquiring enzyme(NAG+LAP),and acid phosphatase(AcP)(P<0.05);after the 13-year restoration,the activities of phenol oxidase(POX)and peroxidase(PER)showed a decreasing tendency during the initial stage of thinning(5 years treatment)and then reverted to pre-thinning values.(3)In the 13-year restoration,thinning significantly increased the soil enzyme carbon-nitrogen ratio(E_(C/N)),soil enzyme carbon-phosphorus ratio(E_(C/P))and vector length value(P<0.05)whereas the E_(C/P)and soil enzyme nitrogen-phosphorus ratio(E_(N/P))increased significantly and the vector angle value decreased in the 5-year restoration(P<0.05).[Conclusion]Soil nutrients,organic carbon,and oxida

关 键 词：森林抚育 土壤胞外酶 酶化学计量 养分限制 

分 类 号：S714[农业科学—林学]