藜麦茎部响应瓜笄霉侵染的代谢组分析  

Metabolomics of the stems of quinoa in response to infection by Choanephora cucurbitarum

作　　者：彭玉飞 王天禧 杨振永 田淼 任璐[1] 吕红 秦楠[1] 殷辉 赵晓军 PENG Yufei;WANG Tianxi;YANG Zhenyong;TIAN Miao;REN Lu;LÜHong;QIN Nan;YIN Hui;ZHAO Xiaojun(College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China)

机构地区：[1]山西农业大学植物保护学院,山西太原030031

出　　处：《微生物学通报》2024年第11期4590-4603,共14页Microbiology China

基　　金：山西省重点研发计划(2022ZDYF117);山西省基础研究计划(20210302123419);山西省现代农业产业技术体系建设专项(2024CYJSTX03-31);“特”“优”农业高质量发展科技支撑工程项目(TYGC24-31,TYGC24-32)。

摘　　要：【背景】藜麦笄霉软腐病(quinoa Choanephora rot)的病原为瓜笄霉(Choanephora cucurbitarum),但藜麦响应其侵染后的代谢变化尚未明确。【目的】研究瓜笄霉侵染藜麦后的代谢物变化及关键代谢途径,以利于了解该病的生理生化机制。【方法】基于非靶向代谢组学研究技术,结合多元统计分析方法,分析瓜笄霉侵染藜麦茎部代谢物差异及代谢途径变化。【结果】相较于对照组,瓜笄霉侵染藜麦后,显著差异代谢物有512种(P<0.01),其中283种为上调,229种为下调。KEGG富集分析发现藜麦茎部响应瓜笄霉侵染的主要差异代谢物共19种(P<0.05),这些主要代谢物显著富集到花生四烯酸代谢、亚油酸代谢、α-亚麻酸代谢、黄酮和黄酮醇的生物合成共4条代谢通路。【结论】瓜笄霉侵染可导致藜麦中花生四烯酸代谢、亚油酸代谢、α-亚麻酸代谢、黄酮和黄酮醇的生物合成等代谢途径发生变化,其中α-亚麻酸代谢及其相关代谢物茉莉酸的积累、黄酮和黄酮醇的生物合成受阻,可能是藜麦响应瓜笄霉侵染的主要方式。[Background]The pathogen responsible for quinoa Choanephora rot is Choanephora cucurbitarum.However,the metabolic changes of quinoa in response to the infection remain unclear.[Objective]We studied the changes of metabolites and key metabolic pathways in quinoa after infection with C.cucurbitarum,aiming to understand the physiological and biochemical mechanisms of the disease.[Methods]We employed non-targeted metabolomics and multivariate statistical analysis to investigate the changes in metabolites and metabolic pathways in quinoa stems after infection with C.cucurbitarum.[Results]Compared with the control,infection with C.cucurbitarum in quinoa resulted in 512 differential metabolites(P<0.01),including 283 upregulated metabolites and 229 downregulated metabolites.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed 19 main differential metabolites in the quinoa stems in response to infection(P<0.05).These differential metabolites were enriched in four metabolic pathways:arachidonic acid metabolism,linoleic acid metabolism,alpha-linolenic acid metabolism,and flavone and flavonol biosynthesis.[Conclusion]C.cucurbitarum infection leads to changes in arachidonic acid metabolism,linoleic acid metabolism,α-linolenic acid metabolism,and flavone and flavonol biosynthesis in quinoa.The accumulation of jasmonic acid fromα-linolenic acid metabolism and the blocked biosynthesis of flavone and flavonol may be involved in the response of quinoa to C.cucurbitarum infection.

关 键 词：藜麦 笄霉软腐病 瓜笄霉 非靶向代谢组学 代谢物 

分 类 号：S435.19[农业科学—农业昆虫与害虫防治]