Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver  

作　　者：Han-Min Wang Yan-Chen Li Lu-Lu Sun Ming-Ye Tang Jia Liu Jiahao Cai Lei Dong Jia Li Yi Zang Hai-Hao Han Xiao-Peng He 

机构地区：[1]Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [2]Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China [3]School of Pharmacy,Weifang Medical University,Weifang 261053,China [4]Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Feringa Nobel Prize Scientist Joint Research Center,Frontiers Center for Materiobiology and Dynamic Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China [5]The International Cooperation Laboratory on Signal Transduction,National Center for Liver Cancer,Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,China [6]Lingang laboratory,Shanghai 201203,China [7]University of Chinese Academy of Sciences,Beijing 100049,China [8]School of Pharmaceutical Science and Technology,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310058,China [9]School of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210023,China

出　　处：《Chinese Chemical Letters》2024年第11期448-453,共6页中国化学快报（英文版）

基　　金：National Natural Science Foundation of China(Nos.22107029,22377135,22305178,92253306 and 82130099);the Taishan Scholars Program(No.tsqn202312305);the Young Elite Scientists Sponsorship Program by Chinese Chemical Society;the Fundamental Research Projects of Science&Technology Innovation and development Plan in Yantai City(No.2023JCYJ059);the Shandong Laboratory Program(No.SYS202205);the Fundamental Research Funds for the Central Universities(No.222201717003);the Programme of Introducing Talents of Discipline to Universities(No.B16017);the Open Funding Project of the State Key Laboratory of Bioreactor Engineering;the State Key Laboratory of Drug Research(No.SKLDR-2023-KF-10);the Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer(Naval Medical University)(No.2023-MEKLLCMS/ZD-00*)。

摘　　要：Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signature of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD.

关 键 词：Lipid droplets imaging Fluorescent probe Large Stokes shift Protein encapsulation Non-alcoholic fatty liver 

分 类 号：O657.3[理学—分析化学] R575.5[理学—化学]