细胞膜仿生金纳米颗粒对膜来源细胞的表型及超微结构的影响  

Effects of Cell Membrane Biomimetic Gold Nanoparticles on the Phenotype and Ultrastructure of Membrane-derived Cells

作　　者：周瑞丽 帅雨汐 齐阔 ZHOU Ruili;SHUAI Yuxi;QI Kuo(The First School of Clinical Medicine,Lanzhou University,Lanzhou 730000,China;Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province,The First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一临床医学院,兰州730000 [2]兰州大学第一医院甘肃省生物治疗与再生医学重点实验室,兰州730000

出　　处：《中国生物工程杂志》2024年第10期29-38,共10页China Biotechnology

基　　金：国家自然科学基金(51801089);甘肃省自然科学基金(22JR11RA017)资助项目。

摘　　要：目的:探究细胞膜仿生金纳米颗粒在和膜来源细胞相互作用过程中对细胞增殖、生长、凋亡等表型及细胞超微结构的影响,评估纳米颗粒和细胞的共存状态。方法:利用混合机械挤压法得到肝星状细胞膜仿生金纳米颗粒(hepatic stellate cell membrane biomimetic gold nanoparticle,AuNP@HSCM);利用高分辨透射电子显微镜表征AuNP@HSCM的纳米-仿生膜互作结构,并对AuNP@HSCM干预前后肝星状细胞LX-2的亚细胞超微结构变化特征进行分析;利用光学显微镜、流式细胞术和CCK-8法检测肝星状细胞LX-2的生长状态、增殖和凋亡等表型特征。结果:AuNP@HSCM的细胞膜和纳米颗粒表面耦合良好;相较于聚乙二醇化的金纳米颗粒(polyethylene glycol modified gold nanoparticle,AuNP@PEG),AuNP@HSCM可输运至细胞核,具有更强的细胞内化能力;与AuNP@PEG引起胞质中细胞器结构退化不同,AuNP@HSCM引发了LX-2细胞近细胞膜处的泡状结构的产生;与AuNP@PEG作用于LX-2细胞相比,AuNP@HSCM在低剂量质量浓度(≤1.5μg/mL)下具有更弱的细胞毒性,能够显著降低LX-2细胞凋亡水平。结论:细胞膜仿生金纳米颗粒AuNP@HSCM具备稳定的纳米-仿生膜耦合结构,与膜来源细胞LX-2能够更好地实现结构和功能共存。Objective:To study the effects of cell membrane biomimetic gold nanoparticles on cell phenotypes such as proliferation,growth and apoptosis as well as cell ultrastructure during the interactions between cell membrane biomimetic gold nanoparticles and membrane-derived cells.We will then evaluate the coexistence status of nanoparticles and cells.Methods:Hepatic stellate cell membrane biomimetic gold nanoparticle(AuNP@HSCM)were prepared using a mixed mechanical extrusion method.High-resolution transmission electron microscope was used to characterize the nano-biomimetic membrane interaction structure of AuNP@HSCM,and to analyze the subcellular ultrastructural changes of LX-2 hepatic stellate cells before and after treatment with [e-mail] microscopy,flow cytometry and the CCK-8 method were used to determine the growth status,proliferation and apoptosis phenotypic characteristics of LX-2 hepatic stellate cells.Results:The cell membrane and nanoparticle surface of AuNP@HSCM are well coupled.Compared with polyethylene glycol-modified gold nanoparticles(AuNP@PEG),AuNP@HSCM can be transported to the cell nucleus and has a stronger cellular internalization ability.Unlike AuNP@PEG,which caused the degradation of organelle structures in the cytoplasm,AuNP@HSCM induced bleb-like structures near the cell membrane in LX-2 cells.Compared with AuNP@PEG acting on LX-2 cells,AuNP@HSCM has weaker cytotoxicity at low dose concentrations(≤1.5μg/mL)and can significantly reduce the apoptosis level of LX-2 cells.Conclusion:Cell membrane biomimetic gold nanoparticles AuNP@HSCM have a stable nano-biomimetic membrane coupling structure,and can better achieve structural and functional coexistence with the membrane-derived cell LX-2.

关 键 词：细胞膜仿生 金纳米颗粒 肝星状细胞 细胞表型 超微结构 

分 类 号：Q813[生物学—生物工程]