分子检测技术在血流感染病原学诊断中的研究进展  

Progress in research of molecular detection techniques in the pathogenetic diagnosis of bloodstream infections

作　　者：赵子今 滑少为 赵萌 申辛欣[3] 马学军[3] 帖彦清[2] Zhao Zijin;Hua Shaowei;Zhao Meng;Shen Xinxin;Ma Xuejun;Tie Yanqing(Hebei North University,Zhangjiakou 075000,Hebei,China;Department of Clinical Laboratory,Hebei General Hospital,Shijiazhuang 050051,Hebei,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Health Commission Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]河北北方学院,河北张家口075000 [2]河北省人民医院检验科,河北石家庄050051 [3]传染病溯源预警与智能决策全国重点实验室国家卫生健康委员会医学病毒和病毒病重点实验室中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《疾病监测》2024年第10期1314-1322,共9页Disease Surveillance

基　　金：国家重点研发计划(No.2021YFC2301102);国家自然科学基金(No.82202593);河北省重点研发计划(No.223777100D)。

摘　　要：血流感染是指由于各种病原微生物(细菌、真菌和病毒等)或毒素进入血流后所引起的全身性播散感染,严重危及人类生命,死亡率极高。现在诊断血流感染的金标准仍为血培养,但检测周期长,阳性率低,无法满足早期血流感染快速诊断的要求,尽早使用有效的抗生素治疗能显著提高患者的生存率。近年来随着分子诊断技术的发展,弥补了血培养的局限性,主要分成了对血培养物中病原菌进行鉴定的分子方法和对全血样本中病原菌进行直接鉴定的分子方法,这些方法在不同场景下各有优势。本研究对目前血流感染病原体分子检测技术的研究进展进行综述。Bloodstream infection(BSI)is a systemic disseminated infection caused by various pathogenic microorganisms(bacteria,fungi,viruses,etc)or toxins entering the bloodstream,which is a serious threat to human life and has a very high mortality rate.Nowadays,the gold standard for the diagnosis of bloodstream infection is still blood culture,but the long testing period and low positive rate cannot meet the requirements of rapid diagnosis of early bloodstream infection,and the early use of effective antibiotic treatment can significantly improve the survival rate of patients.In recent years,with the development of molecular diagnostic technology,which makes up for the limitations of blood culture,it is mainly divided into molecular methods for identifying pathogens in blood cultures and molecular methods for directly identifying pathogens in whole blood samples,which have their own advantages in different scenarios.This study summarizes the progress in the molecular detection of pathogens in BSI.

关 键 词：血流感染 血培养 分子检测技术 研究进展 

分 类 号：R211[医药卫生—中医学] R446