环塔里木盆地2个芦笋品种的代谢组学分析  

Metabolomics study of two different varieties of Asparagus based on LC-MS technique

作　　者：庄红梅[1] 赵家芬 王燕[3] 陈先知[4] 刘会芳[1] 韩宏伟[1] 克丽比努尔·开赛尔 王强[1] 王浩[1] ZHUANG Hongmei;ZHAO Jiafen;WANG Yan;CHEN Xianzhi;LIU Huifang;HAN Hongwei;Kelibinuer Kaisaier;WANG Qiang;WANG Hao(Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences/Xinjiang Vegetable Engineering Research Center/Provincial and Ministerial Co-Construction State Key Laboratory of Crop Stress Resistance Genetic Improvement and Germplasm Innovation in Arid Desert Region/Xinjiang Key Laboratory of Genomic Research and Genetic Improvement of Specialty Fruits and Vegetables,Urumqi 830091,China;Hotan Agricultural Technology Extension Center,Hotan Xinjiang 848000,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Wenzhou Vocational College of Science and Technology,Wenzhou Zhejiang 325006,China)

机构地区：[1]新疆农业科学院园艺作物研究所/新疆蔬菜工程技术研究中心/省部共建干旱荒漠区作物抗逆遗传改良与种质创新国家重点实验室/新疆特色果蔬基因组研究与遗传改良重点实验室,乌鲁木齐830091 [2]和田地区农业技术推广中心,新疆和田848000 [3]南京农业大学园艺学院,南京210095 [4]温州科技职业学院,浙江温州325006

出　　处：《新疆农业科学》2024年第10期2408-2416,共9页Xinjiang Agricultural Sciences

基　　金：天山英才-科技创新领军人才;新疆维吾尔自治区重大科技专项(2022A02005-2);中央高校基本科研业务费专项资金(KYYJ2022001);新疆蔬菜产业技术体系(XJARS-07)。

摘　　要：【目的】芦笋是百合科天门冬属植物石刁柏(Asparagus officinalis)的食用嫩茎,分析2个芦笋品种转录途径和代谢组成分,为深度评估芦笋营养价值和芦笋种质资源开发利用和品种选育提供理论依据。【方法】利用UPLC-MS对哥兰德和翡翠明珠2个芦笋品种的嫩茎并进行代谢组学研究,比较代谢组成分的种类和含量,分析芦笋转录途径和代谢组成分。【结果】共筛选出差异代谢物235种,占全部代谢物的13.03%,其中包括黄酮53种,氨基酸及其衍生物39种,酚酸类34种,木脂素21种,生物碱20种,脂质12种,有机酸8种,核苷酸及其衍生物7种,萜类7种,维生素6种,甾体5种,糖类4种,香豆素3种,酮类2种,醌类1种,醛类1种,以及其它12种。翡翠明珠与哥兰德相比,翡翠明珠的矢车菊素-3-O-半乳糖苷、没食子儿茶素-(4α→8)-没食子儿茶素、丁香苷、芥子碱、2-乙酰基-5-甲基呋喃与槲皮素-3-O-桑布双糖苷含量较高;哥兰德的葡萄糖基5,8-二羟基-2,6-二甲基八碳-2,6-二烯酸,N-阿魏酰尸胺、毛蕊花糖苷、1-O-(6'-O-阿魏酰)葡萄糖苷-3-O-咖啡酰奎尼酸和知母皂苷A3-葡萄糖苷含量较高。【结论】通过富集235种代谢物,得到次生代谢物的生物合成途径主要有氨基酸、辅助因子与黄酮类化合物的生物合成途径。【Objective】Asparagus known as the'king of vegetables'is the edible tender stem of the asparagus officinalis in the lily family,so this project aims to clarify the two varieties of asparagus transcriptional pathway and metabolism components.The results of this study has provided theoretical basis for in-depth evaluation of nutritional value,development and utilization of germplasm resources and variety breeding of asparagus.【Methods】A comparative metabolomic study was carried out on the young stems of two varieties of Galande and Jade Pearl asparagus by UPLC-MS and the transcriptional pathway and metabolic components of asparagus were comprehensively understood by comparing the types and contents of metabolic components.【Results】A total of two hundred and thirty-five different metabolites were screened out accounting for 13.03%of the total metabolites,including fifty-three flavonoids,thirty-nine amino acids and their derivatives,thirty-four phenolic acids,twenty-one lignans,twenty alkaloids,twelve lipids,eight organic acids,seven nucleotides and their derivatives,seven terpenoids,six vitamins,five steroids,four sugars,three coumarins,two ketones,one quinone,one aldehyde,and twelve others.The cyanidin-3-O-galactoside,gallocatechin-(4α→8)-gallocatechin,syringin,sinapine,2-acetyl-5-methylfuran and quercetin-3-O-sambubioside were higher than those of galactoside.Golande had a high content of glucosyl 5,8-dihydroxy-2,6-dimethylocta-2,6-dienoic acid,N-feruloyl cadaverine,acteoside,1-O-(6'-o-feruloyl acid)glucoside,3-O-caffeoyl quinic acid and timosaponin A3-glucoside.【Conclusion】Through enrichment of two hundred and thirty-five kinds of metabolites,the main biosynthesis pathways of secondary metabolites include amino acids,cofactors and flavonoids.

关 键 词：芦笋 LC-MS 代谢 转录途径 

分 类 号：S63[农业科学—蔬菜学]